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Research reports

Dynamics of predators in rice canopy and capacity of natural control on insect pests in paddy fields with different nitrogen regimes ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ L¨¹ Zhong-xian, et al. (225)
ª¤ Effects of Bt-cotton and non-Bt cotton on development and reproduction of Bemisiatabaci (Gennadius) ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ZHOU Fu-cai, et al. (230)
ª¤ Molecular detection and sequence analysis of wsp gene from Wolbachia in two species of pteromalids,Pteromalus puparum and Nasonia vitripennis (Hymenoptera:Pteromalidae) ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­WANG Huan, et al. (235)
ª¤ Resistance of different rice varieties to the striped stem borer, Chilo suppressalis, and its relationship with the morphological and anatomic characteristics of rice¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ XU Hong-xing, et al. (241)
ª¤ Synergistic effect of fermented solution produced by transgenic Beauveria bassiana increa- sing the insecticidal efficacy of wild-type strain results from subtilisin-like activity¡­¡­¡­¡­¡­¡­ZHANG Yong-jun, et al. (246)
ª¤ Effects of transgenic indica rice expressing a gene of cry1Ab with insect resistance on the development and reproduction of nontarget pest, Sogatella furcifera (Homoptera:Delphacidae)¡­¡­¡­¡­¡­¡­¡­TAN Hong, et al. (251)
ª¤ Identification of physiological races and AFLP analysis in F1 generation of sexual hybri- dization between isolates of Setosphaeria turcica¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­HOU Xiao-qiang, et al.(257)
ª¤ Variation in sensitivity of Pythium spp. to metabolite produced by Pseudomonas fluorescent LT6 ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ LOU Bing-gan, et al.(263)
ª¤ Antifungal activity of endophytic fungi isolated from Taxus mairei ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ FU Ke-he, et al. (268)
ª¤ Preparation of antiserum to the coat protein of Scallion mosaic virus and its serological relationships to other potyviruses¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­SHI Yu-hong, et al. (273)
ª¤ The antifungal activity of secondary metabolic products from Xenorhabdus nematophilus YL001 ¡­¡­¡­ LIU Xia, et al. (277)
ª¤ Establishment of a real-time monitoring and forecasting system on main crop diseases and pests of China based on GIS¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ SI Li-li, et al. (282)
ª¤ Inheritance of resistance for Chinese wheat stripe rust races in a new common wheat variety Chuanmai 42 derived from synthetics between Triticum durum¡ÁAegilops tauschii¡­¡­¡­¡­¡­¡­¡­¡­ ZHANG Yong, et al. (287)
ª¤ Alternatives to methyl bromide and reducing its emission technology on strawberry¡­¡­CAO Ao-cheng, et al. (291)
ª¤ Effects of pyrethroid insecticides on chemical communication system of female cotton bollworm, Helicoverpa armigera¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ WEI Hong-yi, et al. (298)
ª¤ Dynamic analysis of peroxidase activity in tobacco leaf induced by quinclorac¡­¡­ DENG Jian-chao, et al. (303)
ª¤ Synergism of rotenone mixed with fipronil and their effectiveness to Phyllotreta striolata¡­¡­WANG Wen-xiang, et al. (307)ª¤
Isolation, purification, identification, and bioassay of helminthosporin with herbicidal activity from Curvularia eragrostidis¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­JIANG Shu-jun, et al. (313)
ª¤ Effects of four fungicides on controlling the powdery mildew and their impact on quality of flos lonicerae, Lonicera japonica Thunb.¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­ NI Yun-xia, et al. (319)ª¤
Bio-activity of propiconazole against larvae and cells of Spodoptera litura ¡­¡­¡­¡­¡­¡­¡­¡­ ZHAO Hai-zhen, et al. (323)
ª¤ Research on application techniques for a novel herbicide 10% ZJ0273 EC on transplanted rapes ¡­¡­TANG Qing-hong, et al. (328)

Notes

Effects of insecticides on glutathione-S-transferase and superoxide dismutase in Liposcelis bostrychophila Badonnel and L.entomophila (Enderlein)¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­CHENG Wei-xia, et al. (333)
ª¤ Effect of MCPA on young plants of different wheat varieties and their physiological indexes ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­LOU Guo-qiang, et al. (335)

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natural control on insect pests in paddy fields with different nitrogen regimes[¿¯£¬ÖÐ]/ÂÀÖÙÏÍ(Õã½­Ê¡Å©Òµ¿ÆѧԺֲÎï±£»¤Óë΢ÉúÎïÑо¿Ëù£¬º¼ÖÝ 310021), ÓáÏþƽ, K L Heong, ºúÝÍ//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª225¡«229

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Abstract: To evaluate reasonably the impact of nitrogen fertilizer on rice-based ecosystem, the main biological characteristic parameters of rice plants and population dynamics of predators in rice canopy were determined in paddy fields applied with 0, 100 and 200 kg/hm2 nitrogen fertilizer, respectively, and the predation capacity of generalist predators on leaf- and planthoppers was also estimated by monitoring the predated number of alternative prey, pupae of fruit fly, Drosophila sp., in a special predation trap. The results showed that plant height, total leaf area and dry straw weight of rice plants in field with 200 kg N/hm2 were markedly higher than that in field with 100 kg N/hm2, however, no obvious difference between two fields was found in the number of tillers and yield of rice. Predators in rice canopy in all three tested fields were dominated by Tetragnatha sp., Araneus sp., Cyrthorhinus lividipennis, Micraspis sp. and Agriocnemis sp. The most abundant Tetragnatha sp. was sampled at booting stage of rice, while the number of Araneus sp. declined and Cyrthorhinus lividipennis, Micraspis sp. and Agriocnemis sp. increased with the growth of rice. Significantly abundant Tetragnatha sp. and Micraspis sp. were recorded in the field without nitrogen fertilizer than those with nitrogen fertilizer. Both number of spider webs and total web area on rice canopy at booting stage were strongly decreased with the increase of nitrogen fertilizer used in the fields. Predation capacity of generalist predators on pupae of fruit fly at booting and milky stages of rice significantly decreased in the fields applied with nitrogen fertilizer compared to that field without nitrogen fertilizer, and the lowest predation capacity was found in the field with 200 kg/hm2 nitrogen fertilizer. It implies that excessive application of nitrogen fertilizer in paddy field maybe weaken the natural control of generalist predators on insect pests.

Key words: Rice canopy; predator; natural control; nitrogen fertilizer

תBt»ùÒòÃ޺ͳ£¹æÃÞ¶ÔÑÌ·ÛÊ­Éú³¤·¢ÓýºÍ·±Ö³µÄÓ°Ïì=Effects of Bt-cotton and non-Bt cotton on development and reproduction of Bemisia tabaci (Gennadius)[¿¯£¬ÖÐ]/Öܸ£²Å(ÑïÖÝ´óѧũѧԺ£¬ÑïÖÝ 225009), ÈÎ˳Ïé, ¶ÅÓèÖÝ, ÖܹðÉú, ÉòæÂ//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª230¡«234

ÕªÒª£ºÔÚʵÑéÊÒºãκʹóÌï×ÔÈ»Ìõ¼þÏ£¬Í¨¹ý¶ÔתBt»ùÒòÃÞ¹ú¿¹22ºÍ³£¹æÃÞÇ×±¾ãôÃÞ3ºÅµÄ¶Ô±ÈÊÔÑéÑо¿£¬Ì½ÌÖÁ½ÖÖÃÞ»¨¶ÔÑÌ·ÛÊ­Éú³¤·¢ÓýºÍ·±Ö³µÄÓ°Ïì¡£½á¹û±íÃ÷£º28¡æºãÎÂÌõ¼þÏ£¬ÔÚ»¨ÁåÆÚÃÞ»¨ÉÏ£¬¹ú¿¹22ÉϵÄBÐÍÑÌ·ÛÊ­·¢ÓýÀúÆÚ(´ÓÂѵ½³É³æÓð»¯)±È³£¹æÃÞÇ×±¾ãôÃÞ3ºÅ¶Ì17.79%¡¢´æ»îÂʸß4.5%¡¢²úÂÑÁ¿¸ß39.62%¡¢´Æ³æÊÙÃü³¤12.14%¡¢ÄÚÙ÷Ôö³¤ÂÊrm ´ó20.18%£»ÔÚÃçÆÚÃÞ»¨ÉÏ£¬¹ú¿¹22ÉϵÄBÐÍÑÌ·ÛÊ­·¢ÓýÀúÆÚ±ÈãôÃÞ3ºÅ¶Ì14.14%¡¢´Æ³æÊÙÃü³¤17.46%¡¢rm ´ó1.47%£¬´æ»îÂʺͲúÂÑÁ¿²îÒì²»ÏÔÖø¡£ÔÚ´óÌï×ÔÈ»±äÎÂÌõ¼þÏ£¬¹ú¿¹22ÉÏÑÌ·ÛÊ­·¢ÓýÀúÆÚ±ÈãôÃÞ3ºÅ¶Ì13.6%¡£ÔÚͬһƷÖÖÃÞ»¨ÉÏ£¬ËÇÑøÔÚÃçÆÚÃÞ»¨ÉÏÑÌ·ÛÊ­µÄ·¢ÓýÀúÆڽϻ¨ÁåÆÚÃÞ»¨³¤¡£½á¹ûÏÔʾ£¬»¨ÁåÆÚÃÞ»¨±ÈÃçÆÚÃÞ»¨¸üÓÐÀûÓÚÑÌ·ÛÊ­µÄÉú³¤·¢ÓýºÍ·±Ö³£»Óë³£¹æÃÞÇ×±¾Ïà±È£¬×ªBt»ùÒòÃÞ»¨ÉÏÑÌ·ÛÊ­µÄÖÖȺÀ©ÔöËÙÂʸü¿ì¡£±í5²Î16

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Abstract: The effects of two type cottons, Bt-cotton Guokang 22 (GK22) and non-Bt cotton Simian 3 (S3), on the development and reproduction of sweet potato whitefly, Bemisia tabaci (Gennadius), were investigated both under lab constant temperature and field inconstant temperature conditions. The results indicated that the developmental time (from egg to adult) of B. tabaci on the GK 22 cotton at flowering stage shortened by 17.79%, the survivorship, fecundity, the life span of female and rm increased by 4.5%, 39.62%, 12.14% and 20.18%, respectively, compared with S3 cotton. At the seedling stage, the developmental time (from egg to adult) of B. tabaci increased by 14.14%, the life of female and rm increased by 17.46% and 1.47%, respectively. And no significant difference was detected in survivorship and fecundity of the whiteflies on two types of cottons. Moreover, under field inconstant temperature conditions, the developmental time of B. tabaci on Goukang No.22 was 13.6% shorter than that on S3 cotton. The cotton plant at flowering stage is more beneficial to the development and reproduction of B. tabaci than that cotton plants at seedling stage. Compare with non-Bt transgenic cotton, Bt transgenic cotton was more advantageous to the development and reproduction of sweet potato whitefly, B. tabaci.

Key words: Bemisia tabaci; rm; Bt-cotton; non-Bt cotton

Á½ÖÖ½ðС·äÌåÄÚWolbachiaµÄwsp»ùÒò·Ö×Ó¼ì²â¼°ÐòÁзÖÎö= Molecular detection and sequence analysis of wsp gene from Wolbachia in two species of pteromalids, Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) [¿¯£¬ÖÐ]/Íõ»¶£¨Õã½­´óѧÀ¥³æ¿ÆѧÑо¿Ëù£¬º¼ÖÝ 310029), Àî¿­£¬Áõ»³£¬ºúÝÍ£¬Ò¶¹§Òø//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª235¡«240

ÕªÒª£º²ÉÓÃWolbachia µÄͨÓÃÒýÎï¼°AºÍB´ó×éÌØÒìÐÔÒýÎï¶ÔµûÓ¼½ðС·äPteromalus puparumºÍÀöÓ¬Ó¼¼¯½ðС·äNasonia vitripennisÌåÄÚWolbachiaµÄwsp»ùÒò½øÐÐPCRÀ©Ôö¼°ÐòÁвⶨ£¬²¢¶Ô²â¶¨µÄÐòÁнøÐÐÁËͬԴÐԱȽϺͻùÒòÌØÕ÷·ÖÎö¡£½á¹û±íÃ÷£ºµûÓ¼½ðС·äºÍÀöÓ¬Ó¼¼¯½ðС·ä¾ù±»AºÍBÁ½¸ö´ó×éµÄWolbachia¸´ºÏ¸ÐȾ£»Í¬ÖÖ¼ÄÉú·äµÄ´Æ·äºÍÐÛ·äµÄwsp»ùÒòƬ¶ÎÐòÁÐÍêÈ«Ò»Ö¡£²ÉÓÃͨÓÃÒýÎï´ÓµûÓ¼½ðС·äÖÐÀ©Ôö³öWolbachiaµÄwsp»ùÒòƬ¶ÎÐòÁеij¤¶ÈΪ540 bp£¬ÊôÓÚB´ó×éÖÐPip×飬¶ø´ÓÀöÓ¬Ó¼¼¯½ðС·äÖÐÀ©Ôö³öWolbachiaµÄwsp»ùÒòƬ¶ÎÐòÁеij¤¶ÈΪ558 bp£¬ÊôÓÚA´ó×éÖÐUni×é¡£ÓÃA-WolbachiaÒýÎï´ÓµûÓ¼½ðС·äºÍÀöÓ¬Ó¼¼¯½ðС·äÖÐÀ©Ôö³öµÄwsp»ùÒòƬ¶ÎÐòÁ㤶ÈΪ548 bp£¬Í¬Ô´ÐÔ´ï99.8£¥£»¶øÓÃB-WolbachiaÒýÎï´ÓÁ½ÕßÖÐÀ©ÔöµÄÁ½Ìõwsp»ùÒòƬ¶ÎÐòÁг¤¶È·Ö±ðΪ424 bpºÍ439 bp£¬Í¬Ô´ÐÔ´ï87.5£¥¡£Í¼3±í2²Î15

¹Ø¼ü´Ê£ºµûÓ¼½ðС·ä£»ÀöÓ¬Ó¼¼¯½ðС·ä£»Wolbachia£»wsp£»»ùÒòÌØÕ÷

Abstract: Polymerase chain reaction (PCR) amplification of a portion of a gene encoding a surface protein of Wolbachia (wsp) was used to detect the Wolbachia infection of the population of Pteromalus puparum and Nasonia vitripennis by universal primers of wsp gene and the specific primers for wsp genes of A and B Wolbachia supergroup. The PCR products were cloned, sequenced and analysed. The results demonstrated that both species were infected with strains of A and B Wolbachia, and wsp gene sequences of females and males from the same wasp were identical. By a pair of universal primers, a region of 540 bp of Wolbachia wsp gene were amplified from both of P. puparum females and males, likewise a region of 558 bp from N. vitripennis. The strains of Wolbachia, which infected P. puparum and N. vitripennis, were classified as the Pip group of B-Wolbachia and Uni group of A-Wolbachia, respectively. Both 548 bp regions of A-Wolbachia were amplified from these two wasps, and the sequence percent identity was 99.8%. In contrast, the 424 bp and 439 bp regions of B-Wolbachia were respectively amplified from P. puparum and N. vitripennis, and their sequence percent identity was 87.5%.

Key words: Pteromalus puparum; Nasonia vitripennis; Wolbachia; wsp; phylogeny

²»Í¬Ë®µ¾Æ·ÖÖ¶Ô¶þ»¯ÃøµÄ¿¹ÐÔ¼°ÆäÓëÐÎ̬ѧºÍ½âÆÊѧÌØÕ÷µÄ¹Øϵ=Resistance of different rice varieties to the striped stem borer,Chilo suppressalis, and its relationship with the morphological and anatomic characteristics of rice [¿¯£¬ÖÐ]/ÐìºìÐÇ£¨Õã½­Ê¡Å©Òµ¿ÆѧԺֲÎï±£»¤Óë΢ÉúÎïÑо¿Ëù£¬º¼ÖÝ310021)£¬ÂÀÖÙÏÍ£¬³Â½¨Ã÷£¬Ö£ÐíËÉ£¬ÓáÏþƽ//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª241¡«245

ÕªÒª£ºÔÚʵÑéÊÒÌõ¼þÏÂÑо¿ÁË6¸ö²»Í¬ÀàÐ͵ÄË®µ¾Æ·ÖÖ¶Ô¶þ»¯ÃøµÄ¿¹ÐÔ¼°ÆäÓëÐÎ̬ѧºÍ½âÆÊѧÌØÕ÷µÄ¹Øϵ¡£½á¹û±íÃ÷£¬¶þ»¯ÃøÓ׳æÔÚ¾¬µ¾Æ·ÖÖÁɾ¬294ºÍÐãË®11ÉϵĴæ»îÂÊ×îµÍ£¬ÏÔÖøµØµÍÓÚÔÚ³¬¼¶ÔÓ½»ô̵¾Á½ÓÅÅà¾ÅºÍÔÓ½»ô̵¾ÉÇÓÅ63ÉϵĴæ»îÂÊ£¬µ«³¬¼¶ÔÓ½»ô̵¾Ð­ÓÅ9308ºÍÔÓ½»ô̵¾ÉÇÓÅ10ÉϵĴæ»îÂÊÎÞÏÔÖø²îÒì¡£60ÈÕÁäʱÉÇÓÅ63µÄ·ÖÞÁÊý×î¶à£¬ÉÇÓÅ10´ÎÖ®£¬Áɾ¬294×îÉÙ¡£ÔÓ½»Ë®µ¾Æ·ÖÖµÄÖê¸ßÏÔÖø¸ßÓÚÆÕͨ¾¬µ¾Æ·ÖÖ£¬¶øÁɾ¬294ºÍÉÇÓÅ63µÄÒ¶ÇÊÏÔÖø¶ÌÓÚÆäËüÆ·ÖÖ¡£³ýЭÓÅ9308ÓëÁ½¸ö¾¬µ¾Æ·ÖÖÎÞÏÔÖø²îÒìÍ⣬ÆäËüÈý¸öÔÓ½»µ¾Æ·ÖÖµÄÒ¶¿íºÍÒ¶½Ç¾ù´óÓÚ¾¬µ¾Æ·ÖÖ¡£60ÈÕÁäʱÔÓ½»ô̵¾Æ·ÖÖÁ½ÓÅÅà¾ÅÒ¶ÇʵÄά¹ÜÊø¼ä¾à×î¿í£¬Áɾ¬294µÄά¹ÜÊø¼ä¾à×îÕ­£¬µ«Æäά¹ÜÊøÖ±¾¶×î´ó¡£Áɾ¬294ºÍÐãË®11Ò¶Çʹèϸ°ûµÄÃܶÈÏÔÖø¸ßÓÚÆäËü¼¸¸öô̵¾Æ·ÖÖ¡£±»¶þ»¯ÃøÓ׳æΣº¦ºó£¬³ýÉÇÓÅ10Ò¶ÇʵÄά¹ÜÊø¼ä¾àÂÔÓÐϽµ£¨-0.28%£©Í⣬ÆäËü¼¸¸öÆ·ÖÖµÄÒ¶ÇÊά¹ÜÊø¼ä¾à¾ùÔö´ó£¬ÆäÖÐÁɾ¬294Ôö·ù×î´ó£¬Îª33.33%¡£¶øά¹ÜÊøÖ±¾¶ºÍ¹èϸ°ûÃܶȾùÓв»Í¬³Ì¶ÈµÄϽµ£¬ÆäÖйèϸ°ûÊýϽµ×îΪÃ÷ÏÔ¡£Í¼2±í2²Î13

¹Ø¼ü´Ê£ºË®µ¾Æ·ÖÖ£»¶þ»¯Ãø£»¿¹ÐÔ£»ÐÎ̬ÌØÕ÷£»½âÆÊѧÌØÕ÷

Abstract: The resistances of six rice varieties, two super-hybrid indica Liangyoupeijiu and Xieyou 9308, two hybrid indica Shanyou 63 and Shanyou 10, and tow Japonica Liaojing 294 and Xiushui 11, to the striped stem borer (SSB), Chilo suppressalis, was monitored in laboratory, and the relationship among resistance and morphological and anatomic characteristics of rice plants were evaluated. Among the six rice varieties tested, the survival rate of newly hatched larvae of SSB on Liaojing 294 and Xiushui 11 were significantly lower than that on Liangyoupeijiu and Shanyou 63, while the survival rate was lower than that on other two hybrid indica cultivars, Xieyou 9308 and Shanyou 10. The number of tillers in Shanyou 63 was highest and the lowest was founded in Liaojing 294. The plant height of hybrid indica varieties was obviously higher than that of Japonica varieties. The same trends were observed in leaf width and leaf angle of rice plant with exception of Xieyou 9308. The space between vascular bundles in leaf sheath of Liangyoupeijiu was the broadest and that in Liaojing 294 was the narrowest. The number of silica cells in leaf sheath of Liaojing 294 and Xiushui 11 were higher than that in four hybrid indica cultivars tested. The spaces between vascular bundles in leaf sheath of the tillers that the main stem was infested by the SSB larvae and caused deadheart, were broader than those in healthy plants with exception of Shanyou 10, and the highest change percent of spaces between vascular bundles was found in Liaojing 294. However, the diameter of vascular bundles and number of silica cells in the sheath decreased. It implies that the infestation of SSB may influence the resistance of rice varieties to SSB.

Key words: Rice varieties; Chilo suppressalis; resistance; morphological character; anatomic character

ת»ùÒòÇòæß°×½©¾ú·¢½Í²úÎï¶ÔÒ°Éú¾úÖêµÄÔöЧ×÷ÓÃ=Synergistic effect of fermented solution produced by transgenic Beauveria bassiana increasing the insecticidal efficacy of wild-type strain results from subtilisin-like activity[¿¯£¬ÖÐ]/ÕÅÓÀ¾ü£¨Å©Òµ²¿ÉúÎï¼¼ÊõÓë×÷ÎïÆ·ÖʸÄÁ¼Öص㿪·ÅʵÑéÊÒ£¬ÖØÇìÊÐÅ©ÒµÉúÎï¼¼ÊõÖصãʵÑéÊÒ£¬Î÷ÄÏ´óѧÉúÎï¼¼ÊõÑо¿ÖÐÐÄ£¬ÖØÇì400716), ½ð¿­, ÂÞÖ¾±ø, ½¯Ð¡¶«, ÅáÑ×//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª246¡«250

ÕªÒª£ºÑо¿·¢ÏÖ£¬³¬Á¿±í´ïÀà¿Ý²Ý¸Ë¾úµ°°×ø»ùÒòCDEP-1µÄÇòæß°×½©¾ú¾úÖêBb0062-15-4-CDEP-1µÄ·¢½Í²úÎï¶ÔÒ°Éú¾úÖê¾ßÓÐÃ÷ÏÔµÄɱÌÒÑÁÔöЧ×÷Ó᣽øÒ»²½·ÖÎöÔöЧ×÷ÓÃÓëÄ¿µÄ»ùÒò±í´ï²úÎï¹ØϵµÄ½á¹ûÏÔʾ£¬¼ÓÈëCDEP-1ø»îΪ44.3¡À0.6 U/¦ÌLµÄ·¢½Í²úÎ¿ÉʹҰÉú¾úÖê¶ÔÌÒÑÁµÄÖÂËÀÖÐŨ¶È½µµÍ4±¶ÒÔÉÏ£¬¹²¶¾ÏµÊý£¨CTC£©´ï404.6¡£µ«·¢½Í²úÎï¾­121¡æ´¦Àí20 min£¬ÔöЧ×÷ÓÃÍêȫɥʧ¡£Óñ¥ºÍÁòËá麟Áµíµ°°×ÖÊ·Ö±ð½«Í¸ÎöºóµÄÖØÈܵ°°×ÖʺÍÉÏÇåÒºÓÃÓÚÔöЧ×÷Óòⶨ£¬½á¹ûÖ¤Ã÷ÔöЧ³É·ÖÖ÷ÒªÊǵ°°×ÀàÎïÖÊ¡£½«ÌݶÈÏ¡Ê͵ķ¢½Í²úÎïÓëŨ¶ÈΪ1¡Á107æß×Ó/mLµÄÒ°Éú¾úÖêæß×ÓÐü¸¡Òº»ìºÏ½øÐÐÉúÎï²â¶¨£¬·¢ÏÖ¸÷´¦ÀíµÄÖÂËÀÖÐʱÓëCDEP-1µÄ»îÐԳʸºÏà¹Ø£¬Ïà¹ØϵÊýΪ-0.8946¡£ÓÉ´ËÍƶϣ¬·¢½Í²úÎïÖеÄÔöЧÎïÖÊÖ÷ÒªÊÇÄ¿µÄ»ùÒò±í´ï²úÎïCDEP-1¡£Í¼3±í1²Î7

¹Ø¼ü´Ê£ºÀà¿Ý²Ý¸Ë¾úµ°°×ø£»Çòæß°×½©¾ú£»·¢½Í²úÎï

Abstract: We found that the ferment product of a transgenic Beauveria bassiana, overexpressing CDEP-1, a cuticle degrading subtilisin-like gene, obviously increased the insecticidal efficacy of wild type strain against aphids Myzus persicae. To investigate the relationship between synergistic effect and CDEP-1 activity in the fermented solution£¬we mixed the ferment product (44.3¡À0.6 U/?L of CDEP-1 activity) with wild type conidia. Bioassay indicated that adding with the ferment product led to a more than 4-fold decrease in the median lethal concentration (LC50) of conidia against aphids, with cotoxicity coefficient (CTC) of 404.6. However, after the solution was heated at 121¡æ for 20 min to quench the protease activity, the synergistic effect almost lost, revealing that the synergistic material in the fermented solution was thermosensitive. When the fermented solution was precipitated by saturated (NH4)2SO4, and then the pellets and the supernatant were dialyzed respectively, and bioassay showed that the synergistic effect existed mainly in the protein part. At the spore concentration of 1¡Á107 /mL, the median lethal time (LT50) of M. persicae was negatively correlated with CDEP-1 activity (the relative coefficient was -0.8946). The results suggested that the synergistic effect of the ferment product mostly resulted from subtilisin-like CDEP-1 activity.

Key words: Subtilisin-like protease; Beauveria bassiana; ferment product

תcry1Ab»ùÒò¿¹³æô̵¾¶Ô·Ç°Ð±êº¦³æ°×±³·ÉÊ­·¢ÓýÓë·±Ö³µÄÓ°Ïì=Effects of transgenic indica rice expressing a gene of cry1Ab with insect resistance on the development and reproduction of nontarget pest, Sogatella furcifera (Homoptera:Delphacidae)[¿¯£¬ÖÐ]/Ì·ºì£¨Õã½­´óѧÀ¥³æ¿ÆѧÑо¿Ëù£¬º¼ÖÝ310029), Ò¶¹§Òø£¬Éò¾ý»Ô£¬ÅíÓÚ·¢£¬ºúÝÍ//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª251¡«256

ÕªÒª£ºÎªÁ˱ȽÏ2¸öתcry1Ab»ùÒò¿¹³æô̵¾Æ·ÏµB1ºÍB6¶ÔÆä·Ç°Ð±êº¦³æ°×±³·ÉÊ­Ãô¸ÐÐÔÓëÆä·Çת»ùÒòÇ×±¾¶ÔÕÕ¼ÎÔç935µÄ²îÒ죬²ÉÓÃÀúÆڲⶨºÍ²úÂÑÐÐΪµç×ӼǼµÈ·½·¨·Ö±ð²â¶¨ÁË°×±³·ÉÊ­ÓÚB1ºÍB6¼°¼ÎÔç935Éϵķ¢ÓýÀúÆÚ¡¢²úÂÑÐÐΪ¼°·±Ö³Á¦¡£½á¹û±íÃ÷£¬¸Ã·ÉÊ­ÒÔB1ºÍB6Ϊʳʱ£¬Æä´ÆÐÛÈô³æ×Ü·¢ÓýÀúÆÚ¾ùÏÔÖø³¤ÓÚ¶ÔÕÕ£¬ÆäÖÐÔÚB1Éϸ÷ÑÓ³¤ÁË1.3ºÍ1.5Ì죬B6ÑÓ³¤ÁË2.0ºÍ1.4Ìì¡£²úÂÑÐÐΪµÄµç×ӼǼ½á¹û±íÃ÷£¬¸Ã·ÉÊ­ÔÚB1ºÍB6ÉϵIJúÂÑƵ´Î¡¢Ò»´Î²úÂÑʱ¼ä¼°×ܲúÂÑʱ¼äÓë¶ÔÕÕÏà±È¾ùÎÞÏÔÖø²îÒì¡£ÔÚ¼ÄÖ÷Ö²Öê¿ÉÑ¡Ôñʱ£¬¸Ã·ÉÊ­ÓÐÆ«°®ÓÚB1ÉϲúÂÑ£¬ÆäÉϵIJúÂѺÛÊý¡¢ÂÑ¿éÊýºÍ×ܲúÂÑÁ¿Ã÷ÏÔ¶àÓÚ¶ÔÕÕ£»¶ø²»ÊȺÃÓÚB6ÉϲúÂÑ£¬ÆäÉϵÄÂÑ¿é¼°ÆäÂÑÁ£Êý¡¢×ܲúÂÑÁ¿Ã÷ÏÔÉÙÓÚ¶ÔÕÕ¡£È»¶ø£¬ÔÚ¼ÄÖ÷Ö²Öê²»¿ÉÑ¡Ôñʱ£¬Ôòδ¹Û²ìµ½ÕâÖÖ²îÒì¡£¾ÍÂÑ·õ»¯ÂʶøÑÔ£¬²»ÂÛÔÚ¼ÄÖ÷¿ÉÑ¡Ôñ»¹ÊDz»¿ÉÑ¡Ôñʱ£¬Óë¶ÔÕÕÏà±ÈB1ºÍB6¶ÔÆä¾ùÎÞÏÔÖøµÄ²»ÀûÓ°Ï졣ͼ2±í3²Î15

¹Ø¼ü´Ê£º×ªcry1Ab»ùÒòô̵¾£»°×±³·ÉÊ­£»·¢ÓýÀúÆÚ£»²úÂÑÐÐΪ£»²úÂÑÁ¿

Abstract: To compare the susceptibility of two homogenous genotypes of transgenic indica rice, namely, B1 and B6 expressing a gene of cry1Ab from Bacillus thuringiensis Berliner against the non-target pest, the white-backed planthopper, Sogatella furcifera (Horvath), with that of their non-transgenic parental cultivar (Jiazao 935), the development duration, oviposition behavior and fecundity of this planthopper on B1 and B6 were measured as compared with those on Jiazao 935 using the methods including a developmental duration measurement, an electronic monitor of the oviposition behavior. The results showed that total developmental time of female and male nymphs were significantly longer on both B1 and B6 than that on the control Jiazao 935, and were increased by 1.3 and 1.5 days for female and male nymphs on B1 and by 2.0 and 1.4 days on B6, respectively. There were no significant differences in the oviposition frequency, the duration per oviposition and total oviposition period among B1, B6 and the control by electronic monitoring system. Under the choice test, the female adults seemingly preferred to lay more eggs on B1 plants rather than on the control, resulting in that more numbers of oviposition scars and egg mass as well as total number of eggs laid were encountered to B1. In contrast, they apparently abstained from laying eggs on B6 plants by comparison with the control, resulting in that less numbers of egg mass and eggs per egg mass as well as total number of eggs laid were observed on B6. However, these significant differences were not found under no-choice test of these plants. As regard to hatching rate, B1 and B6 did not show any obvious adverse effect on it by comparison with the control under either choice or no choice test.

Key words: Transgenic cry1Ab indica rice; Sogatella furcifera; developmental duration; oviposition; fecundity

ÓñÃ×´ó°ß²¡¾úÓÐÐÔÔÓ½»F1´ú¾úÖêµÄÉúÀíСÖÖ¼ø¶¨ºÍAFLP·ÖÎö=Identification of physiological races and AFLP analysis in F1 generation of sexual hybridization between isolates of Setosphaeria turcica [¿¯£¬ÖÐ]/ºîÏþÇ¿(ºÓ±±Å©Òµ´óѧÕæ¾ú¶¾ËØʵÑéÊÒ£¬±£¶¨ 071001), ·¶ÓÀɽ£¬¶­½ð¸Þ£¬Âí¼Ì·¼//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª257¡«262

ÕªÒª£ºÓñÃ×´ó°ß²¡¾úÊÇÒì×ÚÅäºÏÕæ¾ú£¬ÓÐÐÔÔÓ½»ÓпÉÄÜÔöÇ¿²¡¾úµÄÖ²¡Á¦£¬»òÐγÉеÄÖ²¡Ð¡ÖÖ£¬Òò´Ë¶Ô¸Ã²¡¾úÓÐÐÔÔÓ½»ºó´ú½øÐÐÖ²¡ÐԲⶨºÍÒÅ´«¶à̬ÐÔ·ÖÎö¶Ô¿ØÖƸò¡¾úµÄΣº¦¾ßÓÐÖØÒªÒâÒå¡£¶ÔÇ×±¾¾úÖê132¡¢135ºÍËüÃÇÔÓ½»²úÉúµÄ70¸öµ¥×ÓÄÒæß×ÓF1´ú¾úÖê½øÐÐÁËÉúÀíСÖÖ¼ø¶¨ºÍAFLP(À©ÔöÐÔƬ¶Î³¤¶È¶à̬ÐÔ)·ÖÎö¡£ÉúÀíСÖÖ¼ø¶¨½á¹û±íÃ÷£¬F1´ú¾úÖêÖÐÓëÇ×±¾¾úÖê132(23NºÅСÖÖ)ÊôÓÚͬһСÖÖÀàÐ͵ÄÕ¼41.4%£¬ÓëÇ×±¾¾úÖê135(23ºÅСÖÖ)ÏàͬµÄÕ¼20.0%£¬ÁíÍ⻹³öÏÖÁË0¡¢1¡¢2¡¢3¡¢13¡¢123¡¢12N¡¢13NºÍ123NºÅСÖÖ£¬ËùÕ¼±ÈÀý·Ö±ðΪ2.9%¡¢1.4%¡¢2.9%¡¢2.9%¡¢4.3%¡¢8.6%¡¢1.4%¡¢4.3%ºÍ10.0%£¬ËµÃ÷ÓÐÐÔÔÓ½»¿Éʹºó´ú¾úÖêµÄÖ²¡ÐÔ·¢Éú±È½Ï¹ã·ºµÄ±äÒì¡£AFLP·ÖÎö±íÃ÷£¬F1´ú¾úÖêÖ®¼ä·Ö×ÓÒÅ´«ÏàËÆϵÊýÔÚ0.87¡«0.99Ö®¼ä£¬ÆäÖÐ84.3%µÄF1´ú¾úÖêÓëÇ×±¾¾úÖêµÄÒÅ´«ÏàËÆϵÊýÔÚ0.878ÒÔÉÏ£¬µ«ÓëÇ×±¾¾úÖê132ͬԴÐÔ½ÏÇ¿µÄF1´ú¾úÖêÊýÄ¿´óÔ¼ÊÇÓëÇ×±¾¾úÖê135µÄ5±¶£¬ËµÃ÷²»Í¬¾úÖê¾ßÓв»Í¬µÄÒÅ´«´«µÝÄÜÁ¦¡£±È½ÏÉúÀíСÖÖ¼ø¶¨ºÍAFLP·ÖÎö½á¹û£¬·¢ÏÖÉúÀíСÖÖ·Ö»¯ºÍAFLP·Ö×ÓÒÅ´«¶à̬ÐÔ¼äÓÐÒ»¶¨µÄÏà¹ØÐÔ£¬µ«²»ÄÜÍêÈ«¶ÔÓ¦£¬²»´æÔÚÒÅ´«Æ×ϵ¾ÍµÈÓÚСÖֵļòµ¥¶ÔÓ¦¹Øϵ¡£Í¼2±í1²Î17

¹Ø¼ü´Ê£ºÓñÃ×´ó°ß²¡¾ú£»ÓÐÐÔÔÓ½»£»F1´ú¾úÖꣻÉúÀíСÖÖ£»AFLP·ÖÎö

Abstract: Sexual hybridization in Setosphaeria turcica could possibly enhance its pathogenicity and produce new races, therefore, studies on its pathogenicity changes and genetic polymorphism among its descendants will accelerate the control of diseases caused by the fungus. The physiological race identification and AFLP (amplified fragment length polymorphism) analysis were conducted among the 70 single ascospores (F1 isolates) of the sexual hybridization between isolate 132 and isolate 135 of Setosphaeria turcica. The results indicated that 41.4% of F1 isolates were consistent with isolate 132 (race 23N), 20.0% of F1 isolates consistent with isolate 135 (race 23), the other isolates were not consistent with the parent isolates, among which 2.9 % belonged to race 0, 1.4% belonged to race 1, 2.9% belonged to race 2, 2.9% belonged to race 3, 4.3% belonged to race 13, 8.6% belonged to race 123, 1.4% belonged to race 12N, 4.3% belonged to race 13N, and 10.0% belonged to race 123N. The results of physiological race identification among F1 and parent isolates revealed that the sexual hybridization in fungal pathogen could produce diverse variance in pathogenicity. The results of AFLP analysis among F1 and parent isolates indicated that the genetic similarity between F1 isolates was from 0.87 to 0.99. 84.3% of F1 isolates were shown genetically similar to parent isolates with genetic similarity coefficiency of above 0.878, but among which the number of F1 isolates similar to parent isolate 132 was about 5 times of the number similar to parent isolate 135, indicating that different isolates has different ability of genetic transmission. Comparison between the results of physiological race identification and AFLP analysis indicated that there were some correlation between physiological races and DNA polymorphism of the pathogen but not simple genetic pedigree-races relationship between them.

Key words£ºSetosphaeria turcica; sexual hybridization; F1 isolates; physiological race; AFLP

¸¯Ã¹¾ú¶ÔÓ«¹â¼Ùµ¥°û¾úÖêLT6´úлÎïµÄÃô¸ÐÐÔ±äÒì=Variation in sensitivity of Pythium spp. to metabolite produced by Pseudomonas fluorescent LT6 [¿¯£¬ÖÐ]/Â¥±ø¸É(Õã½­´óѧÉúÎï¼¼ÊõÑо¿Ëù£¬º¼ÖÝ 310029)£¬ÁÖîÎ//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª263¡«266

ÕªÒª£ºÎªÁË̽Ã÷²»Í¬°Ð±ê²¡Ô­¾ú¶ÔÉú·À¾úÓ«¹â¼Ùµ¥°û¾úÖêLT6¼°Æä´úлÎïµÄÃô¸ÐÐÔ²îÒ죬ÔÚ1/5 TSAƽ°åÉϲⶨÁËÓ«¹â¼Ùµ¥°û¾úÖêLT6¶Ô69Ö긯ù¾úµÄÞ׿¹Ð§¹û£¬ÔÚÊÔÑéÖй۲쵽¾úÖêLT6¶Ô²»Í¬ÖÖ¸¯Ã¹ÓëͬÖÖ¸¯Ã¹²»Í¬¾úÖêµÄÞ׿¹Ð§¹û´æÔÚÃ÷ÏÔ²îÒ죬ÒÖ¾ú´ø×î¿íµÄΪÖÕ¼«¸¯Ã¹Pythium ultimum¾úÖ꣬´ï7.5 mm, ¶Ôδ֪ÖÖ¸¯Ã¹¾úÖêÒÖ¾ú´ø¿í×îС£¬¾ùСÓÚ3.0 mm¡£¸¯Ã¹¾úÖê¶ÔÓÉLT6²úÉúµÄ¿¹ÉúËØ·Ôàº-1-ôÈËᣨPCA£©µÄÃô¸ÐÐÔ´æÔÚ±äÒ죬±»²âµÄ54Ö긯ù£¬µ±PCAŨ¶ÈΪ7.0¦Ìg/mLʱ£¬Ãô¸ÐµÄΪ28Öê, ¸ß¿¹µÄΪ15Öê¡£Óò»Í¬µÄÅàÑøÒºÅàÑøLT6ËùµÃµÄ´úлÎÔÚÏàͬŨ¶ÈÏ£¬¶Ôͬһ¸¯Ã¹¾ú¾úË¿ÌåÉú³¤µÄÒÖÖÆЧ¹û²»Í¬£¬ÅàÑøÒº1/5 TSBºÍNBÃ÷ÏÔÇ¿ÓÚKMB¡£×ÛºÏËù»ñ½á¹û¿ÉÒÔ¿´³ö£¬°Ð±ê²¡Ô­¾ú¶ÔÉú·À¾úÃô¸ÐÐÔ²»Ò»Ö£»²»Í¬µÄÅàÑø»ù²úÉú²»Í¬µÄ´úл²úÎ¶Ô²¡Ô­¾úµÄÒÖÖÆ×÷ÓÃÓÐÃ÷ÏÔ²îÒì¡£±í3²Î9

¹Ø¼ü´Ê: ¸¯Ã¹£»·Ôàº-1-ôÈË᣻Þ׿¹¾ú£»ÉúÎï·ÀÖÎ

Abstract: In order to investigate the variation in sensitivity of different target pathogens to the biocontrol agent, Pseudomonas fluorescent LT6 and it¡¯s metabolite, the antagonistic effect of Pseudomonas fluorescent LT6 on 69 isolates of Pythium spp. was tested on a 1/5 TSA plate. Strain LT6 exhibited significantly different antagonistic activities against different species of Pythium or different isolates of the same species. It showed the widest inhibition zone against Pythium ultimum (7.5mm)£¬while it was lest effective to unknown species. The size of inhibition zones on those eleven tested isolates were all less than 3.0 mm. The sensitivity of Pythium spp. to PCA (phenazine-1-carboxylic acid), produced by Pseudomonas fluorescent LT6 was variant. When the concentration of PCA was 7.0 ¦Ìg/mL, there were 28 sensitive isolates and 15 highly resistant isolates among the 54 tested isolates. These results demonstrated that the sensitivity of target pathogens to the biocontrol agent varies, and that the metabolite produced in different culture media exhibit distinctly different levels of suppression to pathogens.

Key words: Pythium£»phenazine-1-carboxylic acid£»antagonistic bacteria; biocontrol

ÄÏ·½ºì¶¹É¼ÄÚÉúÕæ¾úµÄ¿¹¾ú»îÐÔ= Antifungal activity of endophytic fungi isolated from Taxus mairei [¿¯£¬ÖÐ]/¸µ¿Æº×£¨Õã½­´óѧÉúÃü¿ÆѧѧԺ£¬º¼ÖÝ 310029£©£¬Õ³õÁú£¬ÁõÊ÷Å³ÂÉÜ襣¬ÁÖ¸£³Ê//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª268¡«272

ÕªÒª£º´ÓÄÏ·½ºì¶¹É¼ÖзÖÀëµ½549ÖêÄÚÉúÕæ¾ú£¬ÒÔÖÕ¼«¸¯Ã¹Pythium ultimum¡¢Á¢¿ÝË¿ºË¾úRhizoctonia solaniΪ°Ð±ê¾ú½øÐп¹¾ú»îÐÔµÄɸѡ¡£½á¹û±íÃ÷£¬¶ÔÖÕ¼«¸¯Ã¹¡¢Á¢¿ÝË¿ºË¾úµÄ°ëÒÖÖÆÏ¡Êͱ¶Êý£¨ID50£©ÔÚ10ÒÔÉϵĻîÐÔ¾úÖê·Ö±ðΪ20Ö꣨ռ×ܾúÖêµÄ3.6%£©ºÍ15Ö꣨ռ×ܾúÖêµÄ2.8%£©¡£ÆäÖа«°ôÇúùAspergillus clavatonanicus¾úÖêF0028¶ÔÕâÁ½ÖÖ²¡Ô­¾úµÄ»îÐÔ×î¸ß£¨¶ÔÖÕ¼«¸¯Ã¹¡¢Á¢¿ÝË¿ºË¾úµÄID50·Ö±ðΪ278¡À15.0¡¢108¡À18.6£©¡£½øÒ»²½Ñо¿±íÃ÷£¬F0028·¢½ÍÒºÔÚpHֵΪ1.0¡«7.0Ö®¼ä¾ùÓнÏÇ¿µÄÒÖ¾ú»îÐÔ£»¾­¸ßθßѹ´¦ÀíºóÒÖ¾ú»îÐÔÈÔ´ïµ½Á˶ÔÕյģ¨65¡À3.8£©%¡£·¢½ÍÒº¾­¸ßЧҺÏàÉ«Æ×´¿»¯µÃµ½5ÖÖ»¯ºÏÎ»îÐԲⶨ±íÃ÷£¬5ÖÖ»¯ºÏÎï¶ÔÖÕ¼«¸¯Ã¹¡¢Á¢¿ÝË¿ºË¾ú¾ùÓв»Í¬³Ì¶ÈµÄÒÖÖÆ×÷Ó㬰ëÒÖÖÆŨ¶È£¨EC50£©ÔÚ7.0¡«45¦Ìg/mLÖ®¼ä¡£Í¼2±í3²Î15

¹Ø¼ü´Ê£ºÄÚÉúÕæ¾ú£»ÖÕ¼«¸¯Ã¹£»Á¢¿ÝË¿ºË¾ú£»°«°ôÇúù£»ÒÖ¾ú»îÐÔ

Abstract: A total of five hundred and forty-nine isolates of endophytic fungi isolated from Taxus mairei were screened for the bioactivity targeted against Pythium ultimum and Rhizoctonia solani. Of 549 isolates, 20 isolates (about 3.6% of the total isolates) showed significant activity against P. ultimum and 15 isolates (about 2.8% of the total isolates) against R. solani with ID50 beyond 10. The isolate F0028, which has the highest inhibitory rate against P. ultimum (ID50 of 278¡À15.0) and R. solani (ID50 of 108¡À18.6) was identified as Aspergillus clavatonanicus. Its crude fermentation broths also showed strong inhibitory activity at pH value range from 1.0 to 7.0 and retained certain inhibitory activity of£¨65¡À3.8)% when autoclaved. Five compounds were isolated from the strain F0028 crude fermentation broths by HPLC. The results of bioassays revealed that all the five compounds have certain inhibitory activity with EC50 between 7.0 and 45 ¦Ìg/mL against two pathogenic fungi.

Key words: Endophytic fungi; Pythium ultimum; Rhizoctonia solani; Aspergillus clavatonanicus; antifungal activity

Þ¯»¨Ò¶²¡¶¾Íâ¿Çµ°°×¿¹ÑªÇåÖƱ¸¼°ÆäÓëÏà¹Ø²¡¶¾ÑªÇåѧ¹Øϵ=Preparation of antiserum to the coat protein of Scallion mosaic virus and its serological relationships to other potyviruses [¿¯£¬ÖÐ]/Ê·Óêºì(Õã½­´óѧÉúÃü¿ÆѧѧԺ£¬º¼ÖÝ 310029)£¬ ³Â¾¼£¬³Â½£Æ½//Ö²Îï±£»¤Ñ§±¨.¡ª2006£¬33£¨3£©.¡ª273¡«276

ÕªÒª£ºÎªÑо¿Þ¯»¨Ò¶²¡¶¾(Scallion mosaic virus, ScaMV)ÓëÆäËüһЩÂíÁåÊíY²¡¶¾Êô³ÉÔ±Ö®¼äµÄѪÇåѧ¹Øϵ£¬Ïȹ¹½¨Ô­ºË±í´ïÔØÌåpSB-ScaMV-CP£¬È»ºóÈ·¶¨¸ÃÖÊÁ£ÄÜÔڴ󳦸˾úBL21 plys S¹ýÁ¿±í´ïÄ¿µÄµ°°×£¬×îºóÓÃÁ½´ÎÖƱ¸SDS-PAGEµçÓ¾·ÖÀë´¿»¯µÄCPÃâÒßСÊ󣬻ñµÃµÄ¿¹ÑªÇåЧ¼ÛΪ1:1024¡£Western blot·ÖÎö±íÃ÷£¬ScaMV CP¿¹ÑªÇåÄÜÓ벡¶¾×ÔÉíCPÓнÏÇ¿µÄÌØÒìÐÔ·´Ó¦£¬ÓëÎßݼ»¨Ò¶²¡¶¾(TuMV)¡¢Ë®ÏÉ»ÆÌõ²¡¶¾(NYSV)¡¢Ð¡Î÷ºù«»Æ»¨Ò¶²¡¶¾(ZYMV)ºÍÓó»¨Ò¶²¡¶¾(DsMV) CPÓнÏÈõµÄ·´Ó¦£¬ÓëÆäËü15ÖÖÂíÁåÊíY²¡¶¾Êô²¡¶¾CPÎÞ·´Ó¦¡£Í¼2²Î14

¹Ø¼ü´Ê: Þ¯»¨Ò¶²¡¶¾£»Ô­ºË±í´ï£»¿¹ÑªÇåÖƱ¸£»Western blot·ÖÎö

Abstract: The complete coat protein gene of Scallion mosaic virus from wild scallion in Hangzhou City, Zhejiang Province was amplified by PCR with specific primers and then inserted into pSBET. The recombinant plasmid of pSB-ScaMV-CP was transformed to BL21plys S, in which the aimed protein was overexpressed after induced by IPTG and then purified by SDS-PAGE. The titre of antiserum obtained in the mice was 1:1024 assayed by indirect ELISA. Based on Western blot analysis, the polycloned antiserum obtained reacted strongly and specifically to its homologous protein. It also reacted less strongly to the prokaryotically expressed CPs of Turnip mosaic virus, Narcissus yellow stripe virus, Zucchini yellow mosaic virus, and Dasheen mosaic virus, but not to other 15 potyviruses.

Key words: Scallion mosaic virus; prokaryotic expression; antiserum preparation; Western blot

ÊÈÏß³æÖ²¡¸Ë¾úYL001¾úÖê´úл²úÎïµÄÒÖ¾ú»îÐÔ=The antifungal activity of secondary metabolic products from Xenorhabdus nematophilus YL001[¿¯£¬ÖÐ]/Áõϼ£¨Î÷±±Å©ÁֿƼ¼´óѧÎÞ¹«º¦Å©Ò©Ñо¿·þÎñÖÐÐÄ£¬ÉÂÎ÷ ÑîÁè 712100), Àîå¹£¬ÍõÓÀºê£¬ÐíÏÍ£¬ÕÅÐË//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª277¡«281

ÕªÒª£º´ÓÉÂÎ÷ÑîÁèÍÁÑùÖÐɸѡµÃµ½Ò»ÖêÀ¥³æ²¡Ô­Ï߳棬´ÓÆäÌåÄÚ·ÖÀëµ½ËüµÄ¹²Éú¾úÊÈÏß³æÖ²¡¸Ë¾úXenorhabdus nematophilus£¬±àºÅΪYL001£¬ÎªÃ÷È·Æä´úл²úÎïµÄÒÖ¾ú»îÐÔ£¬±¾ÊÔÑé²ÉÓÃÒÖÖƾúË¿Éú³¤ËÙÂÊ·¨¡¢ÒÖÖÆæß×ÓÃÈ·¢·¨¡¢×éÖ¯²â¶¨·¨ºÍÅèÔÔÊÔÑ鷨ϵͳ²â¶¨ÁËÆä·¢½ÍÒº¶ÔÖ²ÎﲡԭÕæ¾úµÄÒÖÖÆ×÷Ó᣽á¹û±íÃ÷£¬ÔÚÀëÌåÌõ¼þϸþúÖê·¢½ÍÒº¶Ô¹©ÊÔµÄ15ÖÖÖ²ÎﲡԭÕæ¾ú¾úË¿Éú³¤¾ùÓÐÒ»¶¨µÄÒÖÖÆ×÷Ó㬶Է¬ÇÑ»Òù²¡¾úºÍÀ±½·Òßù²¡¾úµÄÒÖÖÆ×÷ÓÃ×îÇ¿£¬EC50·Ö±ðΪ53.54ºÍ90.39 mg?L-1£»¶Ô¹©ÊÔµÄ6ÖÖÖ²ÎﲡԭÕæ¾úæß×ÓÃÈ·¢¾ùÓÐÒ»¶¨µÄÒÖÖÆ×÷Óã¬ÆäÖжԷ¬ÇÑ»Òù²¡¾úºÍÑ̲ݳàÐDz¡¾úæß×ÓÃÈ·¢ÓнÏÇ¿µÄÒÖÖÆ×÷Óã¬EC50½ö·Ö±ðΪ16.97ºÍ21.87 mg?L-1£»×éÖ¯·¨²â¶¨ÖУ¬·¢½ÍҺϡÊÍ10±¶ºó£¬¶Ô·¬ÇÑ»Òù²¡µÄ±£»¤Ð§¹ûΪ74.98%£¬ÖÎÁÆЧ¹ûΪ63.50%£»¶ÔÅèÔÔ·¬ÇÑ»Òù²¡µÄ±£»¤Ð§¹ûΪ65.11%£¬ÖÎÁÆЧ¹ûΪ54.64%¡£ÏÔ΢¹Û²ì±íÃ÷£¬YL001¾úÖêµÄ·¢½ÍÒº¿Éʹ¾úË¿Éú³¤ÐÎ̬Òì³£¡¢±äÐÎήËõ£¬ÉõÖÁ¶ÏÁÑ¡£Í¼1±í5²Î16

¹Ø¼ü´Ê£ºÊÈÏß³æÖ²¡¸Ë¾ú£»´úл²úÎÒÖ¾ú»îÐÔ£»ÉúÎï²â¶¨

Abstract: This study investigated the antifungal activity of secondary metabolic products from Xenorhabdus nematophilus YL001 in vitro and in vivo. The results indicated that the supernatant of the bacteria broth inhibited mycelial growth of 15-species plant-pathogenic fungal in vitro. It especially could markedly inhibited the mycelial growth to Botrytis cinerea and Phytophthora capsici. Their EC50 value against Botrytis cinerea and Phytophthora capsici were 53.54 mg?L-1 and 90.39 mg?L-1, respectively. The spore germination EC50 value against Botrytis cinerea and Alternaria alternata were 16.97 mg?L-1 and 21.87 mg?L-1, respectively. In addition£¬protective effect and therapeutic effect of the fermentation products diluted 10 times against the Botrytis cinerea were 74.98% and 63.50% on tomato, respectively. Finally protective effect and therapeutic effect of the fermentation products against the Botrytis cinerea were 65.11% and 54.64% on cucumber in potted, respectively. The decrease of spore germination rate and the abnormal hyphal growth was observed under microscope.

Key words£ºXenorhabdus nematophilus; fermentation products; antifungal activity; bioassay

»ùÓÚµØÀíÐÅϢϵͳµÄÈ«¹úÖ÷ÒªÁ¸Ê³×÷Îﲡ³æº¦ÊµÊ±¼à²âÔ¤¾¯ÏµÍ³µÄÑÐÖÆ=Establishment of a real-time monitoring and forecasting system on main crop diseases and pests of China based on GIS [¿¯£¬ÖÐ]/˾ÀöÀö£¨ºÓ±±Å©Òµ´óѧֲÎï±£»¤Ñ§Ôº ºÓ±±Ê¡²¡³æº¦ÉúÎï·ÀÖι¤³Ì¼¼ÊõÑо¿ÖÐÐÄ£¬±£¶¨ 071001)£¬²Ü¿ËÇ¿£¬Áõ¼ÑÅô£¬Ñî¾üÓñ£¬ÕçÎij¬//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª282¡«286

ÕªÒª£ºÎªÌá¸ß²¡³æº¦·ÀÖεÄ׼ȷÐԺͿÆѧÐÔ£¬ÒÀ¾ÝÖ²±£ÖªÊ¶¡¢×¨¼Ò¾­Ñ飬²ÉÓÃÈ˹¤ÖÇÄÜ¡¢×ÔÐпª·¢µÄ¾ßµØÀíÐÅϢϵͳ»ù±¾¹¦Äܼ°¾ö²ßÖ§³Öϵͳ£¨DSS£©µÄÏà¹Ø¼¼Êõ£¬ÑÐÖÆÁË»ùÓÚInternetÍøÂç´«ÊäµÄÈ«¹úÖ÷ÒªÁ¸Ê³×÷Îﲡ³æº¦ÊµÊ±¼à²âÔ¤¾¯ÏµÍ³¡£±¾ÏµÍ³Äܹ»¶ÔСÂó¡¢ÓñÃס¢Ë®µ¾¡¢ÂíÁåÊí¡¢¸ßÁ»ºÍ¹È×Ó6ÖÖÖ÷ÒªÁ¸Ê³×÷ÎïµÄ60ÓàÖÖ²¡³æº¦½øÐÐʵʱ¼à²â¡¢Ô¤¾¯¡¢Õï¶Ï¡¢Ìṩֲ±£ÖªÊ¶£¬Äܹ»¶ÔÄê¶È¼äͬÆÚ²¡Çé¡¢³æÇé×ö³öͼÐαȽϣ¬¿É½«³éÏóµÄÔ¤¾¯Êý¾Ýת»¯³ÉÇåÎú¼òÃ÷µÄµãͼʽµç×ÓµØͼ£¬Ö±¹ÛÃ÷Á˵ØÏÔʾ²¡³æº¦·¢ÉúµãÊý¼°µØÓò·Ö²¼£¬¿É¸ù¾ÝÆ·ÖֵĿ¹²¡³æÐÔ¡¢²¡³æº¦·¢Éú»ùÊýºÍδÀ´ÌìÆøÔ¤±¨µÈÐÅÏ¢¶ÔСÂó°×·Û²¡¡¢³àù²¡¡¢Îƿݲ¡¡¢µ¾ÎÁ²¡¡¢µ¾Çú²¡¡¢ÂíÁåÊíÍíÒß²¡¡¢ÂóÑÁ¡¢Ð¡ÂóÎü½¬³æºÍÓñÃ×Ãø9ÖÖÖØÒª²¡³æº¦×ö³ö¶ÌÆÚ·ÀÖξö²ß£¬ÏµÍ³µÄÔËÐÐÓÐÖúÓÚÌá¸ßÈËÃǶÔÖ÷ÒªÁ¸Ê³×÷Îﲡ³æº¦¹ÜÀíµÄ¿Æѧˮƽ¡£Í¼3²Î12

¹Ø¼ü´Ê£ºÊµÊ±¼à²âÔ¤¾¯£»µØÀíÐÅϢϵͳ£»²¡³æº¦·ÀÖÎ

Abstract: Disease and insect pests are the main limiting factors for crops to increase yield. In order to make the control decision more accurate and more scientific, a real-time monitoring and forecasting system based on internet was established for main crops diseases and insect pests of China, according to knowledge of the plant protection, expert experience, and utilizing artificial intelligence, geography information system (Gsystem1) which was developed by authors and decision support system (DSS) technology. The system could provide the real-time information on occurrence of more than 60 different kinds of diseases and pests in wheat, corn, rice, potato, sorghum and millet of China as well as diagnosing, knowledge providing and short-term controlling decision for nine kinds of important diseases and insect pests, including powdery mildew, sharp eyespot and scab of wheat, rice blast, rice false smut, potato late blight, aphids, grain gall midge of wheat and corn borer, based on crop resistance, primary level of pests and short-term weather forecast. It also had the function of comparing the status of each disease or insect pest during the same period in different years. It can transform data information into a geographical information map, to show the distribution and severity of diseases and pests. The running of the system could help people to control the harmful organisms in a more scientific way.

Key words: Monitoring and forecasting system in real-time; geography information system; pest control

СÂóÐÂÆ·ÖÖ´¨Âó42¿¹ÌõÐⲡÐÔÒÅ´«·ÖÎö=Inheritance of resistance for Chinese wheat stripe rust races in a new common wheat variety Chuanmai 42 derived from synthetics between Triticum durum¡ÁAegilops tauschii[¿¯£¬ÖÐ]/ÕÅïJ£¨ËÄ´¨Å©Òµ´óѧ£¬ÑÅ°² 625014£©£¬ÑîÎäÔÆ£¬ÅíÔÆÁ¼£¬Àî¿¡£¬Ö£ÓÐÁ¼//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª287¡«290

ÕªÒª£ºÌõÐⲡÊÇÎÒ¹úСÂó×îÖØÒªµÄ²¡º¦Ö®Ò»£¬ÑÏÖØÍþвСÂóÉú²ú¡£´¨Âó42ÊÇÀûÓÃÓ²Á£Ð¡Âó-½Ú½ÚÂóÈ˹¤ºÏ³ÉµÄ¸ß¿¹ÌõÐⲡСÂóÐÂÆ·ÖÖ¡£ÎªÃ÷È·´¨Âó42¿¹ÌõÐâÐÔÒÅ´«»ù´¡£¬½«´¨Âó42·Ö±ðÓë¸ß¸ÐÌõÐâСÂóÆ·ÖÖÃàÑô26¡¢ÃàÑô335ÔÓ½»ºÍ»Ø½»£¬»ñµÃÔÓ½»F1¡¢F2¡¢BC1ȺÌå,ÆäÖУ¬´¨Âó42¡ÁÃàÑô26¡¢´¨Âó42¡ÁÃàÑô335 F2ȺÌå·Ö±ðΪ208ºÍ337Öê, ´¨Âó42/ÃàÑô26//ÃàÑô26¡¢´¨Âó42/ÃàÑô335//ÃàÑô335 BC1ȺÌå·Ö±ðΪ171ºÍ216ÖêÓÃÓÚ¿¹ÐÔÒÅ´«·ÖÎö¡£ÀûÓÃÌõÐâ¾úСÖÖÌõÖÐ32ºÅ£¨CYR32£©¶Ô¿¹¸ÐÔÓ½»µÄF1¡¢F2ºÍBC1ȺÌå½ÓÖÖ£¬½á¹ûÏÔʾ£¬ËùÓÐF1´ú¶ÔÌõÖÐ32¶¼±íÏÖÃâÒß»ò¸ß¿¹£¬F2´úȺÌåÖп¹¡Ã¸Ð·ÖÀë±ÈÀý¾ù·ûºÏ3R¡Ã1SÀíÂÛ±ÈÀý£¬BC1ȺÌ忹¡Ã¸Ð·ÖÀë±ÈÒ²·ûºÏ1R¡ÃSÀíÂÛ±ÈÀý£¬ËµÃ÷´¨Âó42¶ÔÌõÖÐ32µÄ¿¹ÐÔÓÉ1¶ÔÏÔÐÔ»ùÒò¿ØÖÆ¡£±í2²Î16

¹Ø¼ü´Ê£ºÐ¡Âó£»È˹¤ºÏ³ÉÖÖ£»´¨Âó42£»ÌõÐⲡ£»¿¹ÐÔÒÅ´«

Abstract: Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases of common wheat, Triticum aestivum L., in China. Chuanmai 42, a new wheat variety derived from synthetics between Triticum durum and Aegilops tauschii, was highly resistant to Chinese newly predominant races of Chinese Yellow Race (CYR32) at adult stage. To determine the inheritance of resistance in Chuanmai 42, it was crossed and backcrossed with the susceptible Sichuan varieties of common wheat Mianyang 26 (MY26) and Mianyang 335 (MY335) for preparing the F2 and BC1 populations. A total of 208 and 337 F2 plants of Chuanmai 42¡ÁMY26£¬Chuanmai 42¡ÁMY335, and 171 and 216 BC1 plants of Chuanmai 42/MY26//MY26£¬Chuanmai 42/MY335//MY335, respectively, were used for resistance analyses. All of F1 hybrids of such crosses were immune or highly resistant and F2, BC1 population displayed the segregation into resistance and susceptible while inoculated with CYR32 (Chinese stripe rust race) in the adult plant stage. The chi-square test confirmed that the frequencies of resistance versus susceptible individuals were in accordance with the 3R:1S and 1R:1S ratio in F2 population and BC1 populations, respectively. The results indicated that the resistance of Chuanmai 42 to CYR32 is controlled by a single dominant gene.

Key words: Wheat; synthetics; Chuanmai 42; Puccinia striiformis f. sp. tritici; resistance inheritance

äå¼×ÍéÔÚ²ÝÝ®ÌïµÄÌæ´ú¼°¼õÉÙÆäÉ¢·¢µÄ¼¼Êõ=Alternatives to methyl bromide and reducing its emission technology on strawberry[¿¯£¬ÖÐ]/²ÜÛê³Ì£¨ÖйúÅ©Òµ´óѧÀíѧԺ£¬±±¾© 100094£©£¬¹ùÃÀϼ£¬¶Îϼ褣¬ÕÅÎļª//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª291¡«297

ÕªÒª£ºäå¼×ÍéÊÇÒ»ÖÖÏûºÄ³ôÑõ²ãµÄÎïÖÊ£¬¸ù¾Ý¡¶ÃÉÌØÀû¶ûÒ鶨Êé¸ç±¾¹þ¸ùÐÞÕý°¸¡·£¬·¢Õ¹Öйú¼Ò½«ÓÚ2015ÄêÌÔÌ­¡£ÔÚÎÒ¹úäå¼×ÍéÏû·ÑÁ¿×î´óµÄµØÇø£¬ºÓ±±Ê¡Âú³ÇÏØʵʩÁËÖÐ-Òâ¡°äå¼×ÍéÍÁÈÀÏû¶¾Ìæ´ú¼¼Êõ¼°ÄÜÁ¦½¨ÉèÏîÄ¿¡±¡£ÊÔÑé½á¹û±íÃ÷£ºÍþ°ÙĶºÍÍþ°ÙĶ+VIF£¬²ÉÓû¯Ñ§¹à¸È¼¼ÊõÊ©Ò©£¬ÊÇÓÐÇ°¾°µÄäå¼×ÍéÌæ´úÆ·£¬2001¡«2002ÄêÍþ°ÙĶºÍÍþ°ÙĶ+VIF´¦Àí¡°´ïÈüÀ³¿ËÌØ¡±Æ·ÖֵIJúÁ¿·Ö±ðΪ26816 kg/hm2ºÍ23672 kg/hm2£¬¶øäå¼×Íé´¦ÀíµÄ²úÁ¿Îª28346 kg/hm2£»Íþ°ÙĶºÍÍþ°ÙĶ+VIF´¦Àí¡°ÀíÄƷÖֵIJúÁ¿·Ö±ðΪ19844 kg/hm2ºÍ15989 kg/hm2£¬¶øäå¼×Íé´¦ÀíµÄ²úÁ¿Îª19657 kg/hm2£»2002¡«2003ÄêÍþ°ÙĶºÍÍþ°ÙĶ+VIF´¦Àí¡°´ïÈüÀ³¿ËÌØ¡±Æ·ÖֵIJúÁ¿·Ö±ðΪ23073 kg/hm2ºÍ23446 kg/hm2£¬¶øäå¼×Íé´¦ÀíµÄ²úÁ¿Îª25285 kg/hm2£»Íþ°ÙĶºÍÍþ°ÙĶ+VIF´¦Àí¡°È«Ã÷ÐÇ¡±Æ·ÖֵIJúÁ¿·Ö±ðΪ£º29594 kg/hm2ºÍ27677 kg/hm2£¬¶øäå¼×Íé´¦ÀíµÄ²úÁ¿Îª32191 kg/hm2¡£²úÁ¿½á¹û¾­Í³¼Æ·ÖÎö±íÃ÷£¬Íþ°ÙĶ¡¢Íþ°ÙĶ+VIFÓëäå¼×Íé¾ùÎÞÏÔÖø²îÒì¡£äå¼×Íé+VIF²ÉÓÃÈÈ·¨Ê©Ò©£¬Æä²úÁ¿Óëäå¼×ÍéÎÞÃ÷ÏÔ²îÒ죬µ«äå¼×Íé+VIFÄܼõÉÙäå¼×Íé25%µÄÓÃÒ©Á¿¡£Ì«ÑôÄÜÏû¶¾+ÉúÎï·ÀÖÎÖƼÁµÄ²úÁ¿¸ßÓڿհ׶ÔÕÕ£¬ÓÐÒ»¶¨µÄ·À²¡Ôö²úЧ¹û£¬µ«ÏÔÖøµÍÓÚäå¼×Íé´¦ÀíµÄ²úÁ¿£¬²»ÄÜ×÷Ϊäå¼×ÍéµÄÌæ´úÆ·¡£½ø¿ÚÆ·ÖÖ¡°ÀíÄºÍµ±µØÖ÷ÔÔÆ·ÖÖ¡°È«Ã÷ÐÇ¡±¶ÔÍÁ´«²¡º¦µÄ¿¹ÐԽϲÔÚ³¤ÊÆ¡¢²úÁ¿·½Ãæ¾ùÏÔÖø²îÓÚÐÂÒý½øÆ·ÖÖ¡°´ïÈüÀ³¿ËÌØ¡±¡£ËäÈ»¡°´ïÈüÀ³¿ËÌØ¡±¶ÔÍÁ´«²¡º¦ÓÐÒ»¶¨µÄ¿¹ÐÔ£¬µ«Óëäå¼×ÍéºÍÍþ°ÙĶ´¦ÀíÇø±È½Ï£¬¿Õ°×¶ÔÕÕÇøµÄ²úÁ¿ÈÔÏÔÖøµÍÓÚÒ©¼Á´¦ÀíÇøµÄ²úÁ¿¡£±í7²Î21

¹Ø¼ü´Ê£ºäå¼×Í飻Íþ°ÙĶ£»Ì«ÑôÄÜÏû¶¾£»¿¹ÐÔÆ·ÖÖ£»²ÝÝ®

Abstract: Methyl bromide is listed as one of the ozone depleting substances (ODS) in the Montreal Protocol and going to be phased out in developing countries in 2015 according to Copenhagen Amendment. A China-Italian cooperative project, Transfer of alternative technologies to the use of methyl bromide and capacity building in soil fumigation (strawberry sector), funded by Italian Ministry for the Environment and Territory was implemented in Mancheng County, Hebei Province. The treatments included metham sodium (MS) 35 g/m2, MS 26.25 g/m2+VIF(virtually impermeable film), solarization+biological control agent (BCA) 10 g/m2, methyl bromide (MB) 50 g/m2 and 55 g/m2 , MB 37.5 g/m2+VIF and MB 41 g/m2+VIF, resistant cultivars and untreated control (CK). The results showed that MS and MS+VIF applied through chemgation were the most promising alternatives to MB. The yields of MS and MS+VIF treatments on c.v. Darselect were 26816 kg/hm2 and 23672 kg/hm2, respectively, while the yield of MB treatment was 28346 kg/hm2. The yield of MS and MS+VIF on c.v. Idea were 19844 kg/hm2 and 15989 kg/hm2, while the yield of MB treatment was 19657 kg/hm2 in 2001-2002. The yields of MS and MS+VIF treatments on c.v. Darselect were 23073 kg/hm2 and 23446 kg/hm2, respectively, while the yield of MB treatment was 25285 kg/hm2. The yield of MS and MS+VIF on c.v. All star were 29594 kg/hm2 and 27677 kg/hm2, while the yield of MB treatment was 32191 kg/hm2 in 2002-2003. There were no significant differences in the tested alternatives from the yield obtained by MB treatment according to statistic analysis. VIF reduced MB emission. Low dosage of MB with VIF by hot gas system did not show any significant difference from normal MB dosage with polyethylene munching in yield and the vigor of strawberry. Solarization + BCA gave higher yield than the untreated control, but it was significantly lower than that from plots treated with MB. It can not be used as an alternative to MB. Resistant c.v. Idea imported from Italy and local c.v. All star did not show good resistance to local soil pathogens, which had more collapsed plants with low yield compared to c.v. Darselect, which showed some resistance to soil pathogens. The yield, however, in MB, MB+VIF, MS, MS+VIF treated plots was higher than that of the untreated ones.

Key words: Methyl bromide; metham sodium; solarization; resistant cultivar; strawberry

Äâ³ý³æ¾Õõ¥Ààɱ³æ¼Á¶ÔÃÞÁå³æ´Æ¶ê»¯Ñ§Í¨Ñ¶ÏµÍ³µÄÓ°Ïì=Effects of pyrethroid insecticides on chemical communication system of female cotton bollworm, Helicoverpa armigera [¿¯£¬ÖÐ]/κºéÒå(½­Î÷Å©Òµ´óѧũѧԺ£¬Äϲý330045)£¬¶Å¼Òγ//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª298¡«302

ÕªÒª£ºÓõÍÖÂËÀ¼ÁÁ¿£¨LD30£©äåÇè¾Õõ¥ºÍÇèÎì¾Õõ¥Ðز¿µãµÎ´¦ÀíÃÞÁå³æ3ÁäÓ׳æºó£¬Ñо¿Äâ³ý³æ¾Õõ¥Ààɱ³æ¼Á¶Ô´æ»î´Æ¶ê»¯Ñ§Í¨Ñ¶ÏµÍ³µÄÓ°Ïì¡£½á¹û±íÃ÷£¬É±³æ¼Á¶Ô´Æ¶êµÄÇóżÐÐΪ²»²úÉúÃ÷ÏÔÓ°Ï죬¶ÔÕÕ×éºÍɱ³æ¼Á´¦Àí×é´Æ¶êµÄÇóż¸ß·å¾ùΪµÚ3¸ö°µÆÚµÄ8¡«8.5 h£¬ÇóżÂʾù¿É´ï73%¡«82%¡£ÇèÎì¾Õõ¥´¦Àí×éÖдƶêÐÔÐÅÏ¢Ëظ÷×é·ÖµÎ¶ÈºÍ×ÜÁ¿Óë¶ÔÕÕÎÞÏÔÖøÐÔ²îÒ죬µ«äåÇè¾Õõ¥´¦Àí×éÖдƶêÐÔÐÅÏ¢ËصÄÖ÷×é·ÖZ11-16¡ÃAldºÍ´ÎÒª×é·ÖZ9-16¡ÃAld¡¢Z7-16¡ÃAldµÄµÎ¶È¼°ÐÅÏ¢ËØ×ÜÁ¿¾ùÏÔÖø¸ßÓÚ¶ÔÕÕ×飬²¢ÇÒÁ½¸ö»îÐÔ×é·ÖZ11-16:AldºÍZ9-16:AldµÄ±ÈÀýÓë¶ÔÕÕ×é´Æ¶êÓÐÏÔÖø²îÒ졣ͼ2±í1²Î19

¹Ø¼ü´Ê£ºÃÞÁå³æ£»»¯Ñ§Í¨Ñ¶ÏµÍ³£»ÐÔÐÅÏ¢ËØ£»äåÇè¾Õõ¥£»ÇèÎì¾Õõ¥

Abstract: The third instar larvae of cotton bollworm, Helicoverpa armigera (H¨¹bner) (Lepidoptera: Noctuidae), were topically treated with pyrethroid insecticides deltamethrin and fenvalerate at dosages causing 30% mortality (LD30) to determine their effects on chemical communication system of the adult survivors. The results showed that pyrethroids had no effect on the calling behaviors of the survivors, and 73%-82% calling behavior appeared at 8-8.5 h after the initiation of darkness at the third scotophase on the treated and untreated females as well. No difference appeared between the titer and percentage of the sex pheromone components in control females and female survived from larvae treated with fenvalerate. However, the titers of major component, Z11-16¡ÃAld, and the minor components, Z9-16¡ÃAld and Z7-16¡ÃAld, as well as the total titers of the sex pheromone blend in the survived female moths treated with deltamethrin were significantly higher than that of the untreated female moths. The ratio of two active components, Z11-16:Ald to Z9-16:Ald in the female moths treated with deltamethrin differed significantly from untreated female moths.

Key words: Helicoverpa armigera; chemical communication system; sex pheromone; deltamethrin; fenvalerate

¶þÂÈà­ßøËáÓÕµ¼Ñ̲ݹýÑõ»¯Îïø»îÐԵĶ¯Ì¬·ÖÎö=Dynamic analysis of peroxidase activity in tobacco leaf induced by quinclorac [¿¯£¬ÖÐ]/µË½¨³¯£¨»ªÄÏÅ©Òµ´óѧÀ¥³æ¶¾ÀíÑо¿ÊÒ, Å©Ò©Ó뻯ѧÉúÎïѧ½ÌÓý²¿ÖصãʵÑéÊÒ£¬¹ãÖÝ 510642)£¬ÍòÊ÷Ç࣬³ÂÔóÅô£¬Õ²ÛàÊÙ£¬Â¬º£²©//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª303¡«306

ÕªÒª£ºÎªÁËÑéÖ¤²»Í¬¼ÁÁ¿¶þÂÈà­ßøËáÖÂÑ̲ݻûÐÎÉú³¤ÓëÓÕµ¼Ö²ÎïÌåÄÚ±£»¤Ã¸ÏµÖеĹýÑõ»¯Îïø»îÐÔ¼äµÄ¹Øϵ£¬²ÉÓÃÅèÔÔÊÔÑéµÄ·½·¨£¬Í¨¹ýÌí¼Ó¶þÂÈà­ßøËᣬģÄâÌï¼ä¶þÂÈà­ßøËáÔÚÍÁÈÀÖеIJ»Í¬²ÐÁôÁ¿£¬Ôì³ÉÑ̲ݲ»Í¬³Ì¶ÈµÄ»ûÐÎÉú³¤, ÔÚ²»Í¬Ê±¼äÄÚ£¬²â¶¨ÑÌÒ¶ÖйýÑõ»¯Îïø£¨POD£©»îÐԵı仯¡£½á¹û±íÃ÷£¬´¦Àí×éÑÌÒ¶ÖÐPODøµÄ»îÐÔÃ÷ÏÔ¸ßÓÚδÓÃÒ©µÄ¶ÔÕÕÑÌÒ¶£¬Ëæ×Å´¦ÀíŨ¶ÈµÄÉý¸ßºÍʱ¼äµÄÑÓ³¤£¬PODøµÄ»îÐÔÓÐÉÏÉýÇ÷ÊÆ£¬µ±´¦Àí28Ììºó£¬¸÷´¦ÀíµÄ¶þÂÈà­ßøËáŨ¶ÈΪ1.04¡Á10-3 ¡¢2.08¡Á10-3¡¢4.17¡Á10-3¡¢ 8.33¡Á10-3ºÍ1.67¡Á10-2mg/kg£¬¶ÔÕպ͸÷´¦ÀíµÄ¹ýÑõ»¯Îïø»îÁ¦·Ö±ðΪ17.47¡¢29.61¡¢23.73¡¢30.33¡¢41.00¡¢57.73 OD?g-1?min-1£¬ÑÌÒ¶ÖйýÑõ»¯Îïø»îÐԽ϶ÔÕÕ·Ö±ðÌá¸ß69.47%¡¢35.80%¡¢73.61%¡¢134.69%ºÍ230.45%¡£PODͬ¹¤Ã¸µÄ¼ì²â±íÃ÷£¬´¦Àí¹ýµÄÑÌҶø´øÔö¶à¡¢ÑÕÉ«¼ÓÖØ¡¢Ã¸»îÔöÇ¿¡£Í¼2²Î12

¹Ø¼ü´Ê£ºÑ̲ݣ»¶þÂÈà­ßøË᣻¹ýÑõ»¯Îïø

Abstract: In order to explain the relationship between the deformity of tobacco caused by quinclorac and the activity of peroxidase, a protective enzyme in the plant induced by the chemical, modeling the dose of quinclorac residue in soil, the seedling of tobacco was transplanted to the soil mixed with quinclorac of different concentrations. The deforming of tobacco growth appeared in different degrees after 7d. Peroxidase (POD) was studied between deformity and normality of tobacco. The results showed that the activity of POD in tobacco leaf treated with quinclorac was obviously higher than that of the control and the activity increased continually with the increasing of concentration in 35 days. When treated with 1.04¡Á10-3£¬2.08¡Á10-3£¬4.17¡Á10-3, 8.33¡Á10-3 and 1.67¡Á10-2 mg/kg, the specific activity of POD was 29.61, 23.73, 30.33, 41.00 and 57.73 OD?g-1? min-1 respectively after 28 days; whereas that of the CK was 17.47OD? g-1?min-1. The rate of activation of POD was 69.47%, 35.80%, 73.61%, 134.69%, 230.45%, respectively. The assay of isoenzymes of POD showed that the enzyme band of deformed leaf treated with quinclorac was more than that of the control, and the color of enzyme band of the deformed leaf was stronger than the control. The relationship of the activity of POD and the growth deformation of tobacco caused by quinclorac was discussed.

Key words: Tobacco; quinclorac; peroxidase

ÓãÌÙͪÓë·ú³æëæ»ìÓÃÔöЧ¶Ô»ÆÇúÌõÌø¼×µÄ¿ØÖÆ×÷ÓÃ=Synergism of rotenone mixed with fipronil and their effectiveness to Phyllotreta striolata[¿¯£¬ÖÐ]/ÍõÎÄÏé(»ªÄÏÅ©Òµ´óѧÀ¥³æ¶¾ÀíÑо¿ÊÒ£¬Å©Ò©Ó뻯ѧÉúÎïѧ½ÌÓý²¿ÖصãʵÑéÊÒ£¬¹ãÖÝ 510642)£¬ÖÓ¹ú»ª£¬Áõºì÷£¬ÎÌȺ·¼£¬ºúÃÀÓ¢//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª307¡«312

ÕªÒª£º»ÆÇúÌõÌø¼×ÊÇÊß²ËÖØÒªº¦³æ£¬Éú²úʵ¼ùÖÐؽÐè¸ßЧ°²È«µÄ·ÀÖÎÒ©¼Á¡£²ÉÓýþ×Õ·¨´ÓÓлúÁס¢Äâ³ý³æ¾Õõ¥¡¢ÐÂÐÍÔÓ»·¡¢Ö²ÎïÔ´ÀàµÈ¹²16ÖÖ³£ÓÃɱ³æ¼ÁÖÐɸѡ³ö¶Ô»ÆÇúÌõÌø¼×³É³æ´¥É±»îÐԽϸߵÄÓãÌÙͪºÍ·ú³æë棬Á½ÕßÒÔ3¡Ã2»ìÓÃʱ£¬´¦Àíºó24 h¶Ô»ÆÇúÌõÌø¼×³É³æµÄLC50Ϊ9.60 ¦Ìg/mL, ¹²¶¾ÏµÊýΪ230.14£¬ÔöЧ×÷ÓÃÃ÷ÏÔ¡£ÒÔ´ËÅä·½ÅäÖƳÉ6%Óã·úÈéÓÍ£¬ÖÊÁ¿Îȶ¨ºÏ¸ñ£¬´¥É±»îÐÔÇ¿£¬×÷ÓÃѸËÙ£¬ÒÔ0.45 ¦Ìg/cm2ºÍ0.75 ¦Ìg/cm2¼ÁÁ¿Ò©Ä¤·¨´¦Àíºó48 hУÕýËÀÍöÂÊ·Ö±ðΪ83.72%ºÍ98.70%£»ÒÔ50 ¦Ìg/mL´¦ÀíÊÔ³æÖÂËÀÖÐʱLT50Ϊ10.12 min¡£Óã·úÈéÓÍ´¦ÀíÏÔÖøÔöÇ¿¶Ô»ÆÇúÌõÌø¼×³É³æºôÎüµÄÒÖÖÆ×÷ÓÿÉÄÜÊÇÆäÖ÷ÒªÔöЧ»úÖÆ¡£6%Óã·úÈéÓÍ450 mL /hm2´¦Àíºó10ÌìÄÚ£¬Ìï¼ä³æ¿Ú¼õÍËÂÊΪ72%¡«96%£¬·ÀÖÎЧ¹ûΪ90%ÒÔÉÏ¡£±í4²Î17

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Abstract: Studies on the synergism of rotenone mixed with fipronil against the striped flea beetle, Phyllotreta striolata (Fab.), were carried out after the investigation of the contact poisoning to the adults of P. striolata with 16 kinds of insecticides, including organophosphorus, pyrethroid, heterocyclic and botanical insecticides. The results in dip tests showed that the value of LC50 was 9.60 ?g/mL and the co-toxicity coefficient was 230.14 after treatment 24 h when ratio of rotenone: fipronil was 3:2. Based on these results, 6% rotenone-fipronil EC was formulated and all of its technical indexes accorded with quality level of pesticide. The adjust mortalities were 83.70% and 98.70% when the treatment dosages were 0.45 ?g/cm2 and 0.75 ?g/cm2 in toxic membrane test after treatment 48 h, respectively. The value of LT50 was only 10.12 min when the adults of P. striolata is treated with 50 ?g/mL in topical application test. The rotenone-fipronil EC could significantly enhanced the breath inhibition to tested adults, which may be the main synergism mechanism. The results of field plot test demonstrated that the amount of adults of P. striolata was reduced by 72%-96% and effectiveness was over 90% when the rotenone-fipronil EC was sprayed with 450 mL/hm2. The rotenone-fipronil EC was worthy to be popularized.

Key words: Rotenone; fipronil; synergism; Phyllotreta striolata

»­Ã¼²ÝÍäæßù¾ú³ý²Ý»îÐÔ»¯ºÏÎïµÄ·ÖÀë¼ø¶¨¼°ÆäÉúÎï»îÐԲⶨ=Isolation,purification, identification,and bioassay of helminthosporin with herbicidal activity from Curvularia eragrostidis[¿¯£¬ÖÐ]/½ªÊö¾ý(ÄϾ©Å©Òµ´óѧÔÓ²ÝÑо¿ÊÒ£¬ÄϾ©210095)£¬Ç¿Ê¤£¬ÖìÔÆÖ¦//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª313¡«318

ÕªÒª£ºÎªÁË·ÖÀë¼ø¶¨ÂíÌÆDigitaria sanguinalis (L.) ScopÉú·À¾ú»­Ã¼²ÝÍäæßùCurvularia eragrostidis¾úÖêQZ-2000µÄÉúÎï»îÐÔÎïÖÊÒÔ¼°ÆÀ¼Û¸ÃÎïÖÊÊÇ·ñ¾ßÓÐ×÷ΪÉúÎïÔ´³ý²Ý¼ÁµÄDZÁ¦£¬ÀûÓÃÉúÎï²â¶¨Îªµ¼ÏòµÄÉ«Æ×·ÖÀë¡¢ÖÊÆ׺ͺ˴Ź²Õñ²¨Æ×¼¼Êõ¶Ô¸Ã¾ú·ÖÃڵĶ¾ËؽøÐÐÁË·ÖÀëºÍ¼ø¶¨£¬ÀûÓÃÖÖ×ÓÃÈ·¢¡¢Å߸ùºÍÑ¿Éú³¤µÄÒÖÖƼ°ÀëÌåҶƬÕë´Ì·¨²â¶¨Á˸ö¾ËضÔÂíÌƵĶ¾º¦×÷Ó㬲¢²â¶¨Á˶¾ËصÄר»¯ÐÔ¼°¶¾ËضÔÂíÌÆÒ¶ÂÌËغ¬Á¿µÄÓ°Ïì¡£½á¹û±íÃ÷£¬¸Ã»¯ºÏÎïΪ½Û»ÆÉ«¾§Ì壬ÈÛ³ÌΪ225¡«227¡æ£¬¾­ÖÊÆ׺ÍNMRÊý¾Ý·ÖÎöÈ·¶¨¸Ã»¯ºÏÎï·Ö×ÓʽΪC15H10O5£¬²¢È·¶¨Îª³¤ÈææßËØ£¨helminthosporin£©¡£¸Ã¶¾ËØÔÚ150¦Ìg/mLŨ¶ÈÏ£¬¶ÔÂíÌÆÅ߸ùÉú³¤ÒÖÖÆÂÊ´ï44.59%£»µ±¶¾ËØŨ¶È´ïµ½200¦Ìg/mLʱ£¬Ò¶ÂÌËغ¬Á¿Ï½µ18.41%£»ÔÚ500¦Ìg/mLŨ¶ÈÏ£¬¶¾ËضԹ©ÊÔµÄ23ÖÖÔӲݲúÉúµÄ¶¾º¦³Ì¶È²»Í¬£¬ÆäÖÐС޼¶Ô¶¾ËØ×îΪÃô¸Ð£¬Æä´ÎΪÂíÌÆ¡¢ÈqÐî¡¢ÈD²Ý¡¢Þ¼ºÍÈÕ±¾¿´ÂóÄïµÈ¶ñÐÔÔӲݡ£ÔÚ¹©ÊÔµÄ5ÖÖ×÷ÎïÖд󶹡¢ÃÞ»¨ºÍ·¬ÇѶԶ¾Ëز»Ãô¸Ð£¬µ«ÓñÃ׺ÍСÂóÔò½ÏΪÃô¸Ð¡£ÉÏÊö½á¹û±íÃ÷£¬³¤ÈææßËØ×÷Ϊ´ó¶¹ºÍÃÞ»¨ÌïÃçºóÉúÎïÔ´³ý²Ý¼ÁÓÐÒ»¶¨µÄ¿ª·¢Ç±Á¦¡£Í¼4±í2²Î16

¹Ø¼ü´Ê£º»­Ã¼²ÝÍäæßù£»³¤ÈææßËØ£»¿¹ÉúËØ£»ÂíÌÆ£»³ý²Ý»îÐÔ

Abstract: The bioassay-guided column chromatography and TLC on silica gel, and MS, 1H- and 13C-NMR spectral techniques were used to isolate and determinate the structure of antibiotic from Curvularia eragrostidis QZ-2000, and to evaluate its herbicidal activity. The herbicidal activity of the toxin to D. sanguinalis was tested by seed germination, the elongation of root and shoot. The needle puncturing leaf assay techniques were used to test the specificity and effect of toxin on photosynthetic pigment content of D. sanguinalis. MS, 1H- and 13C-NMR spectral data indicated that the toxin is helminthosporin. The melting point of the toxin is 225-227¡æ, and the molecular formula is C15H10O5. The toxin had inhibitory effect on elongation of D. sanguinalis root. At 150 ¦Ìg/mL concentration, the inhibition of the toxin to root elongation reached 44.59%. Helminthosporin showed impact on photosynthetic pigment content of D. sanguinalis leaf. When the concentration of toxin reached 200 ¦Ìg/mL, the chlorophyll content of D. sanguinalis leaf decreased by 18.41%. Twenty three troublesome weeds and five crops were tested to study specificity of helminthosporin. The result showed that 18 out of the 28 species were susceptible to the toxin. Chenopodium serotinum is the most susceptive, and D. sanguinalis, Polygonum aviculare, Beckmannia syzigachna, Chenopodium album and Alopecurus japonicus took second place. Soybean, cotton, tomato, Amaranthus retroflexus and Echinochloa crus-galli were resistant to toxin at 500 ¦Ìg/mL concentration. Helminthosporin produced by C. eragrostidis QZ-2000 could be developed as a biocontrol agent for weed control in soybean and cotton fields. 16 Refs. In Chinese.

Key words: Curvularia eragrostidis; Digitaria sanguinalis; helminthosporin; antibiotic; herbicidal activity

ËÄÖÖɱ¾ú¼Á¶Ô½ðÒø»¨°×·Û²¡µÄ·ÀÖÎЧ¹û¼°¶Ô½ðÒø»¨Æ·ÖʵÄÓ°Ïì=Effects of four fungicides on controlling the powdery mildew and their impact on quality of flos lonicerae, Lonicera japonica Thunb.[¿¯£¬ÖÐ]/ÄßÔÆϼ (ºÓÄÏÊ¡Å©Òµ¿ÆѧԺֲÎï±£»¤Ñо¿Ëù£¬Ö£ÖÝ450002£©£¬³ÂÃÀÀ¼£¬ÁõºìÑ壬ÍõÃô£¬»Æè´çù//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª319¡«322

ÕªÒª£º²ÉÓÃËæ»úÇø×éÊÔÑé±È½ÏËÄÖÖɱ¾ú¼Á¶Ô½ðÒø»¨°×·Û²¡µÄ·ÀÖÎЧ¹û£¬ÓøßЧҺÏàÉ«Æ×·¨²â¶¨¸÷´¦ÀíÑùÆ·µÄÂÌÔ­ËẬÁ¿£¬ÒÔɸѡ³ö·ûºÏ½ðÒø»¨¹æ·¶»¯ÖÖÖ²ÐèÒªµÄÒ©¼Á¡£½á¹û±íÃ÷£¬Ê©Ò©ºó14ÌìÒÔ15£¥Èýßòͪ600±¶Òº·ÀÖÎЧ¹û×îºÃ£¬´ï77.8%£¬Æä´ÎÊÇ15£¥Èýßòͪ1200±¶¡¢50£¥¶à¾úÁé500±¶Òº´¦Àí£¬·ÀÖÎЧ¹û·Ö±ðΪ75.7%ºÍ70.8%£¬¼«ÏÔÖøµØ¸ßÓÚ3%ÖÐÉú¾úËØ500ºÍ1000±¶¼°2%Å©¿¹120 1000±¶Òº´¦ÀíµÄ·ÀЧ¡£2£¥Å©¿¹120 500±¶ÒºµÄ·ÀÖÎЧ¹û´ï60.3%£¬ÓëÇ°ÈýÖÖ´¦Àí²îÒì²»ÏÔÖø£¬ÏÔÖøºÃÓÚºóÈýÖÖ´¦Àí£¬µ«Î´´ï¼«ÏÔÖøˮƽ¡£15£¥Èýßòͪ1200±¶·ÀÖδ¦ÀíµÄÂÌÔ­ËẬÁ¿×î¸ß£¬´ï1.683%£¬Æä´ÎÊÇ50%¶à¾úÁé500±¶´¦Àí£¬ÂÌÔ­ËẬÁ¿´ï1.539%£¬·ûºÏ¡¶ÖлªÈËÃñ¹²ºÍ¹úÒ©µä¡·ÒªÇ󣬿É×÷Ϊ½ðÒø»¨¹æ·¶»¯¹ÜÀíÖа׷۲¡·ÀÖÎÓÅÏȲÉÓõÄÒ©¼Á¡£Í¼1±í2²Î10

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Abstract: Randomized block design was used to evaluate the effects of four fungicides on controlling the powdery mildew of the flos lonicerae, Lonicera japonica Thunb., and HPLC was used to determine the content of chlorogenic acid. The results after 14 days of treatment showed that the 15% triadimefon WP at 600 times dilution had best control with 77.8%, followed by 15% triadimefon WP at 1200 times dilution and 50% carbendazim WP at 500 times dilution with the control efficacy of 75.7% and 70.8% respectively, which were the most significantly higher than the control of 3% Zhongshengmycin WP at 500 and 1000 times dilution, and 2% TF-120 SL at 1000 times dilution. The efficacy of 2% TF-120 SL at 500 times solution was 60.3% with no significant difference from that of the former three treatments, but was higher than that of the later three treatments at 5% level of significance. The flos lonicerae treated with 50% carbendazim WP at 500 times dilution had the highest content of chlorogenic acid (1.683%) followed by the treatment (1.539%) with 50% carbendazim WP at 500 times dilution, which could meet the demands of The Pharmacopeia of the PRC. Therefore these two treatments were the first choice for controlling the powdery mildew of flos lonicerae.

Key words: Flos lonicerae; powdery mildew; control; chlorogenic acid

ɱ¾ú¼Á±û»·ßò¶ÔбÎÆÒ¹¶êµÄÉúÎï»îÐÔ=Bio-activity of propiconazole against larvae and cells of Spodoptera litura [¿¯£¬ÖÐ]/ÕÔº£Õä(»ªÄÏÅ©Òµ´óѧÀ¥³æ¶¾ÀíÑо¿ÊÒ£¬Å©Ò©Ó뻯ѧÉúÎïѧ½ÌÓý²¿ÖصãʵÑéÊÒ, ¹ãÖÝ 510642)£¬ÕÅÖ¾Ï飬ÁÎÃÀµÂ£¬Ð캺ºç£¬ÍõÓñ½¡//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª323¡«327

ÕªÒª£ºÎª´ÓÏÖÓÐÅ©Ò©ÖÐÑ°ÕÒɱ³æ¼ÁÏȵ¼»¯ºÏÎÑо¿ÁË11ÖÖÅ©Ò©¶ÔбÎÆÒ¹¶êϸ°ûµÄ¶¾É±»îÐÔ¡£½á¹û±íÃ÷£¬É±¾ú¼Á±û»·ßòµÄ»îÐÔ×î¸ß£¬´¦Àíºó24 hºÍ48 h£¬±û»·ßò¶ÔбÎÆÒ¹¶êϸ°ûµÄLC50Öµ·Ö±ðΪ23.89¦Ìg/mLºÍ13.62 ¦Ìg/mL¡£ÔÚ»îÌåÊÔÑéÖУ¬ÒÔ0.5¦Ìg/Í·¼ÁÁ¿µÄ±û»·ßò×¢ÉäбÎÆÒ¹¶ê4ÁäÓ׳棬´¦Àíºó24 hºÍ48 h£¬ÊÔ³æѪÁÜ°ÍÖÐѪϸ°ûÊý·Ö±ðϽµÁË19.67%¡¢21.38%£¬ÑªÁܰͺ¬Á¿·Ö±ðϽµÁË20.42%¡¢23.87%¡£ÒÔ0.5 ¦Ìg/Í·ºÍ1.0¦Ìg/Í·¼ÁÁ¿µÄ±û»·ßò×¢Éä´¦Àíºó£¬Ð±ÎÆÒ¹¶êÓ׳æÌåÖØÏÔÖø½µµÍ¡£ÔÚ×¢Éä´¦Àíºó48 hºÍ72 h£¬±û»·ßò¶ÔбÎÆÒ¹¶ê4ÁäÓ׳æµÄLD50Öµ·Ö±ðΪ0.91¦Ìg/Í·ºÍ0.63¦Ìg/Í·¡£±û»·ßò¶ÔбÎÆÒ¹¶êϸ°ûºÍбÎÆÒ¹¶êÓ׳æ¾ù¾ßÓнϺõĶ¾É±»îÐÔ£¬ÏÔʾ³öɱ¾ú¼Á±û»·ßòÑÜÉúÎï¿ØÖƺ¦³æµÄ¿ÉÄÜÐÔ¡£±í6²Î9

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Abstract: In order to search lead compound of insecticide, cytotoxicity of 11 kinds of pesticides against Spodoptera litura cells were studied. The results showed that the cytotoxicity of propiconazole was the highest. The LC50 values of propiconazole against S. litura cell were 23.89 ¦Ìg/mL and 13.62 ¦Ìg/mL after treated for 24 h and 48 h respectively. After 0.5 ¦Ìg/larva propiconazole had been injected into the 4th instar larvae of S. litura for 24 h and 48 h, the reduced rates of blood corpuscle of larvae were 19.67% and 21.38%, and the reduced rates of blood content were 20.42% and 23.87% respectively. Furthermore, 0.5 ¦Ìg/larva and 1.0 ¦Ìg/larva propiconazole could depress the larvae weight of S. litura obviously. When treated with propiconazole for 48 h and 72 h, the LD50 values of propiconazole against 4th instar larvae of S. litura were 0.91 ¦Ìg/larva and 0.63 ¦Ìg/larva respectively. It is firstly proved that propiconazole has a high toxicity against S. litura cell and S. litura larva in laboratory, which showed the possibility for propiconazole analog controlling insect pests.

Key words: Propiconazole; Spodoptera litura; cytotoxicity

ÓͲËÌïÐÂÐͳý²Ý¼Á±ûõ¥²ÝÃѵÄÓ¦Óü¼Êõ=Research on application techniques for a novel herbicide 10% ZJ0273 EC on transplanted rapes [¿¯£¬ÖÐ]/ÌÆÇìºì(Öйú¿ÆѧԺÉϺ£Óлú»¯Ñ§Ñо¿Ëù£¬ÉϺ£ 200032)£¬³Â½Ü£¬Éò¹ú»Ô£¬¸¶ÈºÃ·£¬Ç®Õñ¹Ù//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª328¡«332

ÕªÒª£º±ûõ¥²ÝÃÑ£¨ZJ0273£©ÊǾßÓÐÎÒ¹ú×ÔÖ÷֪ʶ²úȨµÄÐÂÐͳý²Ý¼Á£¬ÎªÃ÷È·ÆäÓ¦ÓÃÇ°¾°£¬²ÉÓÃÊÒÍâÅèÔÔ·¨ºÍÎÂÊÒÅèÔÔ·¨£¬ÒÔÓͲËÌï·¢ÉúµÄÖ÷ÒªÔÓ²ÝΪÊԲģ¬¶ÔÐÂÐͳý²Ý¼Á10£¥±ûõ¥²ÝÃÑECɱ²ÝÆס¢Ê©Ò©ÊÊÆÚºÍ×÷ÓÃËٶȵÈÌØÐÔ½øÐÐÁËÑо¿£¬²¢¶Ô×÷ÎïµÄ°²È«ÐԺͳý²ÝЧ¹û½øÐÐÁË×ܽáºÍ·ÖÎö¡£½á¹û·¢ÏÖ£¬¸ÃÒ©¼ÁÔÚ60.0 g ai/hm2¼ÁÁ¿Ï£¬¶Ô¿´ÂóÄï¡¢ÈÕ±¾¿´ÂóÄţ·±ÂÆ¡¢Ð¡Þ¼¡¢ÈD²Ý¡¢Ó²²Ý¡¢ÔçÊìºÌ¡¢°ôÍ·²Ý¡¢´Ì¹ûëݢºÍÜù²ËµÈ10ÖÖÔӲݵķÀЧÔÚ80£¥ÒÔÉÏ£»¸ÃÒ©¼ÁÊôÓÚ»ºÐ§Ðͳý²Ý¼Á£¬ÔÓ²Ý1¡«3Ò¶ÆÚΪʩҩÊÊÆÚ£»±ûõ¥²ÝÃѶԸÊÀ¶ÐÍË«µÍÓͲËÆ·Öֵݲȫ¼ÁÁ¿ÊÇÍƼöÓÃÁ¿µÄ2±¶£»²»Í¬µØÇøÌï¼äʹÓñûõ¥²ÝÃÑ·À³ýÓͲËÌïÔÓ²ÝÓ¦ÔÚÔÓ²Ý1¡«3Ò¶ÆÚÊ©Ò©£¬ÍƼö¼ÁÁ¿Îª45¡«60 g ai/hm2£¬×ÜÌå³ý²ÝЧ¹ûΪ72.3%¡«97.6%¡£±í4²Î7

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Abstract: The control spectrum, suitable application time and action speed of the novel herbicide ZJ0273 were studied with conventional outdoor and greenhouse pot-test methods. The security of herbicide to oilseed rape and controlling effect in field trial were tested. The results showed that the sensitive weeds to ZJ0273 at the application rate of 60 g ai/hm2 were mainly gramineous weeds, such as Alopecurus aequalis, A. japonicus, Malachium aquaticum, Chenopodium serotinum, Poa annua, Sclerochloa kengiana, Beckmannia syzigachne, Polypogon fugax, Ranunculus japonicus and Capsella buras-pastoris with the efficacy over 80%. The herbicide was a slow-acting herbicide, and the suitable application time was in early 1-3 leaf postemergence stage of the weeds. The safe dosage to Huyou No.15 (Brassica napus L.), a new rapeseed variety, doubled the recommended maximum dosage. An application dosage of 45-60 g ZJ0273 ai/hm2 in early 1-3 leaf postemergence stage of the weeds was recommended, and the total weed-control effect was among 72.3%-97.6%.

Key words: ZJ0273; weed control spectrum; application techniques; herbicide in rape field ¡¡¡¡

ËÄÖÖɱ³æ¼Á¶ÔÁ½ÖÖÊéÊ­¹Èë׸ÊëÄ-S-תÒÆøºÍ³¬Ñõ»¯ÎïÆ绯øµÄÓ°Ïì=Effects of insecticides on glutathione-
S-transferase and superoxide dismutase in Liposcelis bostrychophila Badonnel and L.entomophila (Enderlein) [¿¯£¬ÖÐ]/³Ìΰϼ£¨Î÷ÄÏ´óѧֲÎï±£»¤Ñ§Ôº À¥³æѧÓ뺦³æ¿ØÖƹ¤³ÌÖØÇìÊÐÖصãʵÑéÊÒ£¬ÖØÇì 400716)£¬²ñÓñöΣ¬Íõ½ø¾ü//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª333¡«334

2¼×4ÂȶԲ»Í¬Ð¡ÂóÆ·ÖÖÓ×Ãç¼°ÉúÀíÖ¸±êµÄÓ°Ïì=Effect of MCPA on young plants of different wheat varieties and their
physiological indexes[¿¯£¬ÖÐ]/¦¹úÇ¿£¨ºÓÄϿƼ¼Ñ§ÔºÖ²Îï±£»¤Ïµ£¬ÐÂÏç453003)£¬ÂÀÎÄÑå, Ö°Ã÷ÐÇ//Ö²Îï±£»¤Ñ§±¨. ¡ª2006£¬33£¨3£©.¡ª335¡«336¡¡¡¡ ¡¡¡¡